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| Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp. | |
Chen, Xiaolin1,2,3; Song, Lin4 ; Wang, Hui5; Liu, Song1,2,3; Yu, Huahua1,2,3; Wang, Xueqin1,2,3; Li, Rongfeng1,2,3; Liu, Tianzhong5; Li, Pengcheng1,2,3
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| 2019-01-02 | |
| 发表期刊 | MOLECULES
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| ISSN | 1420-3049 |
| 卷号 | 24期号:2页码:11 |
| 通讯作者 | Li, Pengcheng(pcli@qdio.ac.cn) |
| 摘要 | Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols. However, the polysaccharides of Tribonema sp. and their biological activities have rarely been reported. In this paper, we extracted sulfated polysaccharides from Tribonema sp. (TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells. The results showed that TSP is a sulfated polysaccharide with a M-w of 197 kDa. TSP is a heteropolysaccharide that is composed mainly of galactose. It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor (TNF-). In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 mu g/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells. These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP. |
| 关键词 | characterization immune-modulatory anticancer sulfated polysaccharide Tribonema sp |
| DOI | 10.3390/molecules24020322 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Shandong Key Research and Development Project[2016YYSP010] ; Shandong Natural Science Foundation[ZR2017BD019] ; Key Laboratory of Natural Product Chemical Biology, Ministry of Education, Shandong University[CB-201708] ; Shandong Key Research and Development Project[2016YYSP010] ; Shandong Natural Science Foundation[ZR2017BD019] ; Key Laboratory of Natural Product Chemical Biology, Ministry of Education, Shandong University[CB-201708] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS记录号 | WOS:000457137200108 |
| 出版者 | MDPI |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160710 |
| 专题 | 实验海洋生物学重点实验室 海洋生物技术研发中心 |
| 通讯作者 | Li, Pengcheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 通讯作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Chen, Xiaolin,Song, Lin,Wang, Hui,et al. Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.[J]. MOLECULES,2019,24(2):11. |
| APA | Chen, Xiaolin.,Song, Lin.,Wang, Hui.,Liu, Song.,Yu, Huahua.,...&Li, Pengcheng.(2019).Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp..MOLECULES,24(2),11. |
| MLA | Chen, Xiaolin,et al."Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.".MOLECULES 24.2(2019):11. |
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| Partial Characteriza（1379KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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