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Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis
Ning, Junhao1,2,3,4; Song, Chengwen1,2,3; Luo, Danli1,3,4; Liu, Yuan1,2,3; Liu, Hourong1,3,4; Cui, Zhaoxia1,2,3
2019-02-15
Source PublicationGENE
ISSN0378-1119
Volume685Pages:12-20
Corresponding AuthorCui, Zhaoxia(zhxcui@qdio.ac.cn)
AbstractThe maternal genome directs almost all aspects of early animal development. As development proceeds, the elimination of maternal gene products and zygotic genome activation (ZGA) occur during the maternal to zygotic transition (MZT). To study the molecular mechanisms regulating this developmental event in Eriocheir sinensis, RNA-Seq technology was applied to generate comprehensive information on transcriptome dynamics during early embryonic stages. In total, 32,088 annotated unigenes were obtained from the transcriptomes of fertilized eggs and embryos at the cleavage (2-4 cell) and blastula stage. A total of 566 maternal genes and 1165 zygotic genes were isolated, among which 103 and 266 genes were predicted conserved maternal transcripts (COMATs) and conserved zygotic transcripts (COZYTs), respectively. The COMATs performed housekeeping gene functions and may be essential for initiating early embryogenesis of the Bilateria. Furthermore, 87, 76 and 117 differentially expressed genes associated with the MZT, morphogenesis and immunity were identified when compared the three transcriptomic datasets. We also unmask that the MZT takes place around the cleavage stage, when the genes involved in the clearance of maternal gene products and the ZGA were significantly up-regulated. Taken together, these datasets provide a valuable resource for understanding the mechanisms of early developmental events in E. sinensis, and facilitate further studies on molecular mechanisms of asynchronous development in crabs.
KeywordMitten crabs Embryogenesis Maternal to zygotic transition Transcriptomics
DOI10.1016/j.gene.2018.10.036
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of Shandong Province[ZR2017QD001] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000455692300002
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160707
Collection实验海洋生物学重点实验室
Corresponding AuthorCui, Zhaoxia
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ning, Junhao,Song, Chengwen,Luo, Danli,et al. Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis[J]. GENE,2019,685:12-20.
APA Ning, Junhao,Song, Chengwen,Luo, Danli,Liu, Yuan,Liu, Hourong,&Cui, Zhaoxia.(2019).Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis.GENE,685,12-20.
MLA Ning, Junhao,et al."Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis".GENE 685(2019):12-20.
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