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Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis
Ning, Junhao1,2,3,4; Song, Chengwen1,2,3; Luo, Danli1,3,4; Liu, Yuan1,2,3; Liu, Hourong1,3,4; Cui, Zhaoxia1,2,3
2019-02-15
发表期刊GENE
ISSN0378-1119
卷号685页码:12-20
通讯作者Cui, Zhaoxia(zhxcui@qdio.ac.cn)
摘要The maternal genome directs almost all aspects of early animal development. As development proceeds, the elimination of maternal gene products and zygotic genome activation (ZGA) occur during the maternal to zygotic transition (MZT). To study the molecular mechanisms regulating this developmental event in Eriocheir sinensis, RNA-Seq technology was applied to generate comprehensive information on transcriptome dynamics during early embryonic stages. In total, 32,088 annotated unigenes were obtained from the transcriptomes of fertilized eggs and embryos at the cleavage (2-4 cell) and blastula stage. A total of 566 maternal genes and 1165 zygotic genes were isolated, among which 103 and 266 genes were predicted conserved maternal transcripts (COMATs) and conserved zygotic transcripts (COZYTs), respectively. The COMATs performed housekeeping gene functions and may be essential for initiating early embryogenesis of the Bilateria. Furthermore, 87, 76 and 117 differentially expressed genes associated with the MZT, morphogenesis and immunity were identified when compared the three transcriptomic datasets. We also unmask that the MZT takes place around the cleavage stage, when the genes involved in the clearance of maternal gene products and the ZGA were significantly up-regulated. Taken together, these datasets provide a valuable resource for understanding the mechanisms of early developmental events in E. sinensis, and facilitate further studies on molecular mechanisms of asynchronous development in crabs.
关键词Mitten crabs Embryogenesis Maternal to zygotic transition Transcriptomics
DOI10.1016/j.gene.2018.10.036
收录类别SCI
语种英语
资助项目Natural Science Foundation of Shandong Province[ZR2017QD001] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Natural Science Foundation of Shandong Province[ZR2017QD001] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS研究方向Genetics & Heredity
WOS类目Genetics & Heredity
WOS记录号WOS:000455692300002
出版者ELSEVIER SCIENCE BV
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/160707
专题实验海洋生物学重点实验室
通讯作者Cui, Zhaoxia
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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GB/T 7714
Ning, Junhao,Song, Chengwen,Luo, Danli,et al. Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis[J]. GENE,2019,685:12-20.
APA Ning, Junhao,Song, Chengwen,Luo, Danli,Liu, Yuan,Liu, Hourong,&Cui, Zhaoxia.(2019).Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis.GENE,685,12-20.
MLA Ning, Junhao,et al."Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis".GENE 685(2019):12-20.
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