Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Changes in key enzyme activities and metabolites during in vitro maturation of Apostichopus japonicus oocyte under desiccation stress | |
Liu, Shilin1,2,3; Lin, Chenggang1,2,3; Qu, Hongxia4; Sun, Jingchun1,2,3 ; Ru, Xiaoshang1,2,3; Sun, Lina1,2,3; Zhang, Libin1,2,3; Zhang, Tao1,2,3; Zhou, Yi1,2,3; Yang, Hongsheng1,2,3
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| 2019-02-01 | |
| 发表期刊 | AQUACULTURE RESEARCH
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| ISSN | 1355-557X |
| 卷号 | 50期号:2页码:400-411 |
| 通讯作者 | Yang, Hongsheng(hshyang@qdio.cn.cn) |
| 摘要 | Understanding the physiological changes of oocyte in vitro maturation are important for improving in in vitro fertilization rates and embryonic development. In this study, Apostichopus japonicus gonads were dissected, and the oocytes and gonadal fluid extracted from the gonads were dried in darkness to induce oocyte maturation. The changes in the germinal vesicle breakdown percentage (GVBDP), superoxide dismutase (SOD) activity, and the concentrations of glutathione (GSH), cyclic adenosine monophosphate (cAMP), adenylyl cyclase (AC), protein kinase C (PKC), oestradiol (E2), and metabolites in gonadal fluid-oocyte complexes (GFOC) were detected during desiccation. The GVBDP peaked at 85% after 2 hr of desiccation. The SOD activity and GSH concentration in GFOC increased significantly during desiccation, reaching their maximum values at 0.5 and 2 hr of desiccation respectively (p < 0.05). The concentrations of cAMP, AC, PKC, and E2 decreased significantly during the desiccation treatment (p < 0.05) and reached relatively stable levels after 1 hr of desiccation. After 2 hr of desiccation, among the 68 metabolites detected in the GFOC, the concentrations of 52 metabolites increased and those of 16 metabolites decreased. This study not only provides an effective and simple technique for in vitro induction of A. japonicus oocyte maturation under desiccation stress, but also improves our understanding of gamete biology in this species. |
| 关键词 | drying enzymology GVBD metabolome sea cucumber |
| DOI | 10.1111/are.13895 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Agricultural Seed Project of Shandong Province[2017LZGC010] ; Agricultural Seed Project of Shandong Province[2016LZGC032] ; National Natural Science Foundation of China[41606171] ; National Natural Science Foundation of China[41676136] ; Qingdao Applied Basic Research Program[17-1-1-49-JCH] ; Taishan Scholars Program ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1] ; Agricultural Seed Project of Shandong Province[2017LZGC010] ; Agricultural Seed Project of Shandong Province[2016LZGC032] ; National Natural Science Foundation of China[41606171] ; National Natural Science Foundation of China[41676136] ; Qingdao applied basic research program[17-1-1-49-JCH] ; Taishan Scholars Program ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1] |
| WOS研究方向 | Fisheries |
| WOS类目 | Fisheries |
| WOS记录号 | WOS:000455230900004 |
| 出版者 | WILEY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160665 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Yang, Hongsheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Rushan Fisheries Technol Extens Stn, Rushan, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Shilin,Lin, Chenggang,Qu, Hongxia,et al. Changes in key enzyme activities and metabolites during in vitro maturation of Apostichopus japonicus oocyte under desiccation stress[J]. AQUACULTURE RESEARCH,2019,50(2):400-411. |
| APA | Liu, Shilin.,Lin, Chenggang.,Qu, Hongxia.,Sun, Jingchun.,Ru, Xiaoshang.,...&Yang, Hongsheng.(2019).Changes in key enzyme activities and metabolites during in vitro maturation of Apostichopus japonicus oocyte under desiccation stress.AQUACULTURE RESEARCH,50(2),400-411. |
| MLA | Liu, Shilin,et al."Changes in key enzyme activities and metabolites during in vitro maturation of Apostichopus japonicus oocyte under desiccation stress".AQUACULTURE RESEARCH 50.2(2019):400-411. |
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| Changes in key enzym（644KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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