IOCAS-IR  > 海洋生态与环境科学重点实验室
Changes in key enzyme activities and metabolites during in vitro maturation of Apostichopus japonicus oocyte under desiccation stress
Liu, Shilin1,2,3; Lin, Chenggang1,2,3; Qu, Hongxia4; Sun, Jingchun1,2,3; Ru, Xiaoshang1,2,3; Sun, Lina1,2,3; Zhang, Libin1,2,3; Zhang, Tao1,2,3; Zhou, Yi1,2,3; Yang, Hongsheng1,2,3
2019-02-01
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume50Issue:2Pages:400-411
Corresponding AuthorYang, Hongsheng(hshyang@qdio.cn.cn)
AbstractUnderstanding the physiological changes of oocyte in vitro maturation are important for improving in in vitro fertilization rates and embryonic development. In this study, Apostichopus japonicus gonads were dissected, and the oocytes and gonadal fluid extracted from the gonads were dried in darkness to induce oocyte maturation. The changes in the germinal vesicle breakdown percentage (GVBDP), superoxide dismutase (SOD) activity, and the concentrations of glutathione (GSH), cyclic adenosine monophosphate (cAMP), adenylyl cyclase (AC), protein kinase C (PKC), oestradiol (E2), and metabolites in gonadal fluid-oocyte complexes (GFOC) were detected during desiccation. The GVBDP peaked at 85% after 2 hr of desiccation. The SOD activity and GSH concentration in GFOC increased significantly during desiccation, reaching their maximum values at 0.5 and 2 hr of desiccation respectively (p < 0.05). The concentrations of cAMP, AC, PKC, and E2 decreased significantly during the desiccation treatment (p < 0.05) and reached relatively stable levels after 1 hr of desiccation. After 2 hr of desiccation, among the 68 metabolites detected in the GFOC, the concentrations of 52 metabolites increased and those of 16 metabolites decreased. This study not only provides an effective and simple technique for in vitro induction of A. japonicus oocyte maturation under desiccation stress, but also improves our understanding of gamete biology in this species.
Keyworddrying enzymology GVBD metabolome sea cucumber
DOI10.1111/are.13895
Indexed BySCI
Language英语
Funding ProjectAgricultural Seed Project of Shandong Province[2017LZGC010] ; Agricultural Seed Project of Shandong Province[2016LZGC032] ; National Natural Science Foundation of China[41606171] ; National Natural Science Foundation of China[41676136] ; Qingdao applied basic research program[17-1-1-49-JCH] ; Taishan Scholars Program ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000455230900004
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160665
Collection海洋生态与环境科学重点实验室
Corresponding AuthorYang, Hongsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
4.Rushan Fisheries Technol Extens Stn, Rushan, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Shilin,Lin, Chenggang,Qu, Hongxia,et al. Changes in key enzyme activities and metabolites during in vitro maturation of Apostichopus japonicus oocyte under desiccation stress[J]. AQUACULTURE RESEARCH,2019,50(2):400-411.
APA Liu, Shilin.,Lin, Chenggang.,Qu, Hongxia.,Sun, Jingchun.,Ru, Xiaoshang.,...&Yang, Hongsheng.(2019).Changes in key enzyme activities and metabolites during in vitro maturation of Apostichopus japonicus oocyte under desiccation stress.AQUACULTURE RESEARCH,50(2),400-411.
MLA Liu, Shilin,et al."Changes in key enzyme activities and metabolites during in vitro maturation of Apostichopus japonicus oocyte under desiccation stress".AQUACULTURE RESEARCH 50.2(2019):400-411.
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