SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri

doi:10.1038/s41598-018-35983-3

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	SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri
	Liang, Shaoshuai 1,2; Liu, Danwen 1; Li, Xixi 1; Wei, Maokai 1; Yu, Xiaohan 1; Li, Qi 1; Ma, Huixin 1; Zhang, Zhifeng 1; Qin, Zhenkui 1
	2019-01-11
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	9 页码:13
通讯作者	Zhang, Zhifeng(zzfp107@ouc.edu.cn) ; Qin, Zhenkui(qinzk@ouc.edu.cn)
摘要	As an important transcription factor, SOX2 involves in embryogenesis, maintenance of stem cells and proliferation of primordial germ cell (PGC). However, little was known about its function in mature gonads. Herein, we investigated the SOX2 gene profiles in testis of scallop, Chlamys farreri. The level of C. farreri SOX2 (Cf-SOX2) mRNA increased gradually along with gonadal development and reached the peak at mature stage, and was located in all germ cells, including spermatogonia, spermatocytes, spermatids and spermatozoa. Knockdown of Cf-SOX2 using RNAi leaded to a mass of germ cells lost, and only a few spermatogonia retained in the nearly empty testicular acini after 21 days. TUNEL assay showed that apoptosis occurred in spermatocytes. Furthermore, transcriptome profiles of the testes were compared between Cf-SOX2 knockdown and normal scallops, 131,340 unigenes were obtained and 2,067 differential expression genes (DEGs) were identified. GO and KEGG analysis showed that most DEGs were related to cell apoptosis (casp2, casp3, casp8), cell proliferation (samd9, crebzf, iqsec1) and spermatogenesis (htt, tusc3, zmynd10, nipbl, mfge8), and enriched in p53, TNF and apoptosis pathways. Our study revealed Cf-SOX2 is essential in spermatogenesis and testis development of C. farreri and provided important clues for better understanding of SOX2 regulatory mechanisms in bivalve testis.
DOI	10.1038/s41598-018-35983-3
收录类别	SCI
语种	英语
资助项目	Shandong Provincial Natural Science Foundation, China[ZR2016CQ24] ; National High Technology Research and Development Program of China[2012AA10A402] ; Shandong Provincial Natural Science Foundation, China[ZR2016CQ24] ; National High Technology Research and Development Program of China[2012AA10A402]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000455470600022
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/160621
专题	中国科学院海洋研究所
通讯作者	Zhang, Zhifeng; Qin, Zhenkui
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266000, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liang, Shaoshuai,Liu, Danwen,Li, Xixi,et al. SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri[J]. SCIENTIFIC REPORTS,2019,9:13.
APA	Liang, Shaoshuai.,Liu, Danwen.,Li, Xixi.,Wei, Maokai.,Yu, Xiaohan.,...&Qin, Zhenkui.(2019).SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri.SCIENTIFIC REPORTS,9,13.
MLA	Liang, Shaoshuai,et al."SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri".SCIENTIFIC REPORTS 9(2019):13.

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