SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri
Liang, Shaoshuai1,2; Liu, Danwen1; Li, Xixi1; Wei, Maokai1; Yu, Xiaohan1; Li, Qi1; Ma, Huixin1; Zhang, Zhifeng1; Qin, Zhenkui1
Corresponding AuthorZhang, Zhifeng( ; Qin, Zhenkui(
AbstractAs an important transcription factor, SOX2 involves in embryogenesis, maintenance of stem cells and proliferation of primordial germ cell (PGC). However, little was known about its function in mature gonads. Herein, we investigated the SOX2 gene profiles in testis of scallop, Chlamys farreri. The level of C. farreri SOX2 (Cf-SOX2) mRNA increased gradually along with gonadal development and reached the peak at mature stage, and was located in all germ cells, including spermatogonia, spermatocytes, spermatids and spermatozoa. Knockdown of Cf-SOX2 using RNAi leaded to a mass of germ cells lost, and only a few spermatogonia retained in the nearly empty testicular acini after 21 days. TUNEL assay showed that apoptosis occurred in spermatocytes. Furthermore, transcriptome profiles of the testes were compared between Cf-SOX2 knockdown and normal scallops, 131,340 unigenes were obtained and 2,067 differential expression genes (DEGs) were identified. GO and KEGG analysis showed that most DEGs were related to cell apoptosis (casp2, casp3, casp8), cell proliferation (samd9, crebzf, iqsec1) and spermatogenesis (htt, tusc3, zmynd10, nipbl, mfge8), and enriched in p53, TNF and apoptosis pathways. Our study revealed Cf-SOX2 is essential in spermatogenesis and testis development of C. farreri and provided important clues for better understanding of SOX2 regulatory mechanisms in bivalve testis.
Indexed BySCI
Funding ProjectShandong Provincial Natural Science Foundation, China[ZR2016CQ24] ; National High Technology Research and Development Program of China[2012AA10A402]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000455470600022
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorZhang, Zhifeng; Qin, Zhenkui
Affiliation1.Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266000, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liang, Shaoshuai,Liu, Danwen,Li, Xixi,et al. SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri[J]. SCIENTIFIC REPORTS,2019,9:13.
APA Liang, Shaoshuai.,Liu, Danwen.,Li, Xixi.,Wei, Maokai.,Yu, Xiaohan.,...&Qin, Zhenkui.(2019).SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri.SCIENTIFIC REPORTS,9,13.
MLA Liang, Shaoshuai,et al."SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri".SCIENTIFIC REPORTS 9(2019):13.
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