Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions | |
Liu, Chenlin1,2; Zhao, Xia3 ; Wang, Xiuliang2,4
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| 2018-12-01 | |
| Source Publication | JOURNAL OF APPLIED PHYCOLOGY
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| ISSN | 0921-8971 |
| Volume | 30Issue:6Pages:3519-3528 |
| Corresponding Author | Liu, Chenlin(ch.lliu@163.com) |
| Abstract | Heat shock protein DnaK (Hsp70) can prevent irreversible protein denaturation by chaperoning the unfolded polypeptides under extremely cold environment, and enhance cold and freezing tolerance of microorganism. From an Antarctic sea-ice green alga Chlamydomonas sp. ICE-L, eight Hsp70 genes were identified and divided into seven subfamilies: four in the cytoplasmic subfamilies, two in the chloroplast subgroups, and one in the mitochondria or endoplasmic reticulum subfamilies. Phylogenetic analyses showed that except CidnaK, each of the other Cihsp70 genes had their homologs in Chlamydomonas reinhardtii and Volvox carteri. CiDnak is a cytoplasmic protein with highly homologous to DnaK proteins from prokaryotes, and it had 100% amino acid sequence identities with the DnaK of Psychroflexus torquis ATCC 700755, a psychrophilic bacterium isolated from Antarctic sea ice. The transcription of CidnaK was significantly induced upon freezing stress in ICE-L, eminently higher than that of other Cihsp70 genes. In addition, the transformed C. reinhardtii 137c with CidnaK gene showed much higher rates of survival and growth than the wild type under freezing or low temperature conditions. Our results suggested that CidnaK was likely originated from the symbiotic bacterium in the sea-ice brine, and is an important candidate freezing inducible gene that confers low temperature tolerance to Chlamydomonas cells, and plays a crucial role in ICE-L adapting the frigid Antarctic environments. |
| Keyword | Antarctic sea-ice Chlamydomonas Chlorophyta DnaK/Hsp70 Horizontal gene transfer Freezing tolerance |
| DOI | 10.1007/s10811-018-1492-4 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation[41276203] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances |
| WOS Research Area | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS Subject | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS ID | WOS:000455405900052 |
| Publisher | SPRINGER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/160618 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu, Chenlin |
| Affiliation | 1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Qingdao Univ, Coll Environm Sci & Engn, Qingdao 266000, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Chenlin,Zhao, Xia,Wang, Xiuliang. Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions[J]. JOURNAL OF APPLIED PHYCOLOGY,2018,30(6):3519-3528. |
| APA | Liu, Chenlin,Zhao, Xia,&Wang, Xiuliang.(2018).Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions.JOURNAL OF APPLIED PHYCOLOGY,30(6),3519-3528. |
| MLA | Liu, Chenlin,et al."Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions".JOURNAL OF APPLIED PHYCOLOGY 30.6(2018):3519-3528. |
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