IOCAS-IR
Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi)
Dong, Junjian1; Chen, Zhihang3; Sun, Chengfei1,2; Tian, Yuanyuan1; Hu, Jie1; Lu, Maixin1,2; Ye, Xing1,2
2019-02-01
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
卷号228页码:10-16
通讯作者Ye, Xing(gzyexing@163.com)
摘要Myosin heavy chains (MYHs) play important roles in muscle growth and contraction. In fish, MYHs contribute to hyperplasia and hypertrophy of muscle fibers, which can continue into adult life and thus result in indeterminate growth in some species. We previously identified two MYH genes, MYH-7a and MYH-7b, that are differentially expressed in Mandarin fish (Siniperca chuatsi) and appear to function in early growth. However, the regulatory role of their 5' flanking regions is unknown. To examine the effects of single nucleotide polymorphisms (SNPs) in these regions, we used genome walking to amplify their flanking sequences and analyzed the regulatory elements and binding sites. A single SNP locus was found in the flanking sequence of each gene. These SNP loci are located in the conserved glucocorticoid receptor binding region (MYH-7a G-614A; Allele frequency: G:A = 94.9:5.1; GG (89.76) and AG (10.24) genotypes) and the LIM homeobox domain transcription factor binding sequence (MYH-76: C-1933A; Allele frequency: C:A = 54.8:45.2; AA (20.82), AC (48.81), and CC (30.37) genotypes). At the G-614A loci, the GG genotype exhibited more superior growth traits (total length, body length, body height, etc.) than the AG genotype, with the exception of caudal peduncle length. Alternatively, at the C-1933A loci, the AC and AA genotypes showed significant differences in all growth traits, except for head length, with AC exhibiting superior traits. The AA and CC genotypes showed significant differences in caudal peduncle length and height, while no differences were observed between the AC and CC genotypes. Thus, these SNPs in the 5' flanking regions of MYH-7a and MYH-7b are correlated with superior growth and can be used for selecting Mandarin fish during breeding.
关键词5' flanking region Growth correlation analysis Mandarin fish (Siniperca chuatsi) Myosin heavy chain gene (MYH-7) Single nucleotide polymorphism (SNP)
DOI10.1016/j.cbpb.2018.10.006
收录类别SCI
语种英语
资助项目Central Public-interest Scientific Institution Basal Research Fund CAFS[2017HY-ZC0402] ; China Agriculture Research System[CARS-46] ; Ocean Fisheries Science and Technology Promotion Project of Guangdong Province[A201601A06] ; Science and Technology Project of Guangdong Province[2015A020209034] ; Central Public-interest Scientific Institution Basal Research Fund CAFS[2017HY-ZC0402] ; China Agriculture Research System[CARS-46] ; Ocean Fisheries Science and Technology Promotion Project of Guangdong Province[A201601A06] ; Science and Technology Project of Guangdong Province[2015A020209034]
WOS研究方向Biochemistry & Molecular Biology ; Zoology
WOS类目Biochemistry & Molecular Biology ; Zoology
WOS记录号WOS:000456639400002
出版者ELSEVIER SCIENCE INC
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/160612
专题中国科学院海洋研究所
通讯作者Ye, Xing
作者单位1.Chinese Acad Fishery Sci, Pearl River Fisheries Inst, Key Lab Trop & Subtrop Fisheries Resource Applica, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Dong, Junjian,Chen, Zhihang,Sun, Chengfei,et al. Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2019,228:10-16.
APA Dong, Junjian.,Chen, Zhihang.,Sun, Chengfei.,Tian, Yuanyuan.,Hu, Jie.,...&Ye, Xing.(2019).Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,228,10-16.
MLA Dong, Junjian,et al."Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 228(2019):10-16.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Dong, Junjian]的文章
[Chen, Zhihang]的文章
[Sun, Chengfei]的文章
百度学术
百度学术中相似的文章
[Dong, Junjian]的文章
[Chen, Zhihang]的文章
[Sun, Chengfei]的文章
必应学术
必应学术中相似的文章
[Dong, Junjian]的文章
[Chen, Zhihang]的文章
[Sun, Chengfei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。