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Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus
Chen, Xiaowu1,2,3; He, Yudong1; Wang, Zhipeng1; Li, Jun4,5
2018-12-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume226Pages:85-90
Corresponding AuthorLi, Jun(Junli@qdio.ac.cn)
AbstractCytochrome P450 aromatase (P450arom), which is encoded by cyp19a1a, can convert androgen to estrogen. Therefore, P450arom is important in gonadal differentiation and maintenance. In this study, we analyzed the expression and DNA methylation of cypl9a from Chinese sea perch Lateolabrax maculatus (sp. cyp19a1a). The sp. cyp19a1a gene consists of 9 exons, but only 3.5 kb, being smaller than the human cyp19a1a, as a result of small introns. The sp. cyp19a1a protein contains 518 amino acid residues and evolutionarily conserved domains and is clustered in the teleost subfamily on the phylogenetic tree. Amino acid alignment indicates that sp. cyp19a1a shares the highest identity (91.6%) to Epinephelus akaara and Lates calcarifer. Endogenous sp. cyp19a1a is detected mainly in stromal cells around the oocytes of stage I ovary, and the gene expression level has no difference after 40 days fresh water culture in both ovary and testis. The sp. cyp19a1a can catalyze the production of estrogen from androgen in vitro. Seven CpG dinucleotides are found in the proximal promoter. Binding sites of the conserved predicted transcription factors include cAMP response element, steroidogenic factor-1, and SRY-Box. The deletion of this region reduces promoter activity significantly. The methylation level of the seven CpG dinucleotides in cyp19a1a promoter is higher in the testis (44.25 +/- 4.04) than in the ovary (24.71 +/- 3.05). The induced hypermethylation of the sp. cyp19a1a promoter suppressed promoter transcription function in vitro. These results suggest that DNA methylation may be a mechanism used for natural sex maintenance.
Keywordcyp19a1a DNA methylation Expression pattern Lateolabrax maculatus Promoter
DOI10.1016/j.cbpb.2018.07.008
Indexed BySCI
Language英语
Funding ProjectChina Agriculture Research System[CARS-47] ; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2017NO2]
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS IDWOS:000447114400010
PublisherELSEVIER SCIENCE INC
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160476
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Jun
Affiliation1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China
2.Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China
3.Shanghai Collaborat Innovat Aquat Anim Genet & Br, Shanghai 201306, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 26000, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 26000, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Xiaowu,He, Yudong,Wang, Zhipeng,et al. Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2018,226:85-90.
APA Chen, Xiaowu,He, Yudong,Wang, Zhipeng,&Li, Jun.(2018).Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,226,85-90.
MLA Chen, Xiaowu,et al."Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 226(2018):85-90.
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