Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus

doi:10.1016/j.cbpb.2018.07.008

IOCAS-IR > 实验海洋生物学重点实验室

	Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus
	Chen, Xiaowu 1,2,3; He, Yudong 1; Wang, Zhipeng 1; Li, Jun 4,5
	2018-12-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	226 页码:85-90
通讯作者	Li, Jun(Junli@qdio.ac.cn)
摘要	Cytochrome P450 aromatase (P450arom), which is encoded by cyp19a1a, can convert androgen to estrogen. Therefore, P450arom is important in gonadal differentiation and maintenance. In this study, we analyzed the expression and DNA methylation of cypl9a from Chinese sea perch Lateolabrax maculatus (sp. cyp19a1a). The sp. cyp19a1a gene consists of 9 exons, but only 3.5 kb, being smaller than the human cyp19a1a, as a result of small introns. The sp. cyp19a1a protein contains 518 amino acid residues and evolutionarily conserved domains and is clustered in the teleost subfamily on the phylogenetic tree. Amino acid alignment indicates that sp. cyp19a1a shares the highest identity (91.6%) to Epinephelus akaara and Lates calcarifer. Endogenous sp. cyp19a1a is detected mainly in stromal cells around the oocytes of stage I ovary, and the gene expression level has no difference after 40 days fresh water culture in both ovary and testis. The sp. cyp19a1a can catalyze the production of estrogen from androgen in vitro. Seven CpG dinucleotides are found in the proximal promoter. Binding sites of the conserved predicted transcription factors include cAMP response element, steroidogenic factor-1, and SRY-Box. The deletion of this region reduces promoter activity significantly. The methylation level of the seven CpG dinucleotides in cyp19a1a promoter is higher in the testis (44.25 +/- 4.04) than in the ovary (24.71 +/- 3.05). The induced hypermethylation of the sp. cyp19a1a promoter suppressed promoter transcription function in vitro. These results suggest that DNA methylation may be a mechanism used for natural sex maintenance.
关键词	cyp19a1a DNA methylation Expression pattern Lateolabrax maculatus Promoter
DOI	10.1016/j.cbpb.2018.07.008
收录类别	SCI
语种	英语
资助项目	China Agriculture Research System[CARS-47] ; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2017NO2] ; China Agriculture Research System[CARS-47] ; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2017NO2]
WOS研究方向	Biochemistry & Molecular Biology ; Zoology
WOS类目	Biochemistry & Molecular Biology ; Zoology
WOS记录号	WOS:000447114400010
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/160476
专题	实验海洋生物学重点实验室
通讯作者	Li, Jun
作者单位	1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China 2.Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China 3.Shanghai Collaborat Innovat Aquat Anim Genet & Br, Shanghai 201306, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 26000, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 26000, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chen, Xiaowu,He, Yudong,Wang, Zhipeng,et al. Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2018,226:85-90.
APA	Chen, Xiaowu,He, Yudong,Wang, Zhipeng,&Li, Jun.(2018).Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,226,85-90.
MLA	Chen, Xiaowu,et al."Expression and DNA methylation analysis of cyp19a1a in Chinese sea perch Lateolabrax maculatus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 226(2018):85-90.

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