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Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)
Xue, Rui1,2,3,4; Wang, Xueying1,2,4; Xu, Shihong1,2,4; Liu, Yifan1,2,4; Feng, Chengcheng1,2,4; Zhao, Chunyan1,2,4; Liu, Qinghua1,2,4; Li, Jun1,2,4
2018-12-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume226Pages:53-63
Corresponding AuthorLiu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
AbstractEgg yolk generation is a common physiological process in oviparous animals. To understand oogenesis and reproductive capacity, it is necessary to characterize vitellogenins (Vtgs), which are the precursors of major egg yolk proteins (Yps). Therefore, to improve our understanding of the entire process of egg yolk generation in female turbot (Scophthalmus maximus), we obtained full-length cDNAs of vtg genes, examined gene expression in the female liver and ovary, and analyzed Vtg synthesis in the ovary. Three distinct complete polypeptide sequences were identified and designated as VtgAa, VtgAb, and VtgC, which confirmed the multiplicity of the vtg gene in turbot and showed that it follows a "three vtg model". The expression of these three vtg genes in the female liver was far higher than that in other tissues, including the ovary. The expression of all three vtg genes was extremely low before vitellogenesis, and then increased and was maintained at a high level until the degradation stage, which was in accordance with changes in the concentration of estradiol-17 beta (E-2) and the gonadosomatic index. Compared with the liver, the ovary had a higher E-2 level and lower vtg expression, suggesting that some other factors limit high vtg expression in the ovary of turbot. Transcripts of vtgAb and the Yps derived from them were both detected in oogonia and primary oocytes, which showed that these might possess the ability to perform autosynthesis of yolk. These findings add to our understanding of the reproductive physiology of Vtg synthesis in turbot.
KeywordAutosynthesis Estradiol-17 beta Turbot (Scophthalmus maximus) Vitellogenin Yolk protein
DOI10.1016/j.cbpb.2018.08.002
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; China Postdoctoral Science Foundation[2016M592259] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS (2014) ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; improved Seeds Engineering Project of Shandong Province (2014-2017)
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS IDWOS:000447114400007
PublisherELSEVIER SCIENCE INC
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160461
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Qinghua; Li, Jun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xue, Rui,Wang, Xueying,Xu, Shihong,et al. Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2018,226:53-63.
APA Xue, Rui.,Wang, Xueying.,Xu, Shihong.,Liu, Yifan.,Feng, Chengcheng.,...&Li, Jun.(2018).Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,226,53-63.
MLA Xue, Rui,et al."Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 226(2018):53-63.
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