Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus) | |
Xue, Rui1,2,3,4; Wang, Xueying1,2,4 ; Xu, Shihong1,2,4; Liu, Yifan1,2,4; Feng, Chengcheng1,2,4; Zhao, Chunyan1,2,4; Liu, Qinghua1,2,4; Li, Jun1,2,4
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| 2018-12-01 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
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| ISSN | 1096-4959 |
| 卷号 | 226页码:53-63 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 摘要 | Egg yolk generation is a common physiological process in oviparous animals. To understand oogenesis and reproductive capacity, it is necessary to characterize vitellogenins (Vtgs), which are the precursors of major egg yolk proteins (Yps). Therefore, to improve our understanding of the entire process of egg yolk generation in female turbot (Scophthalmus maximus), we obtained full-length cDNAs of vtg genes, examined gene expression in the female liver and ovary, and analyzed Vtg synthesis in the ovary. Three distinct complete polypeptide sequences were identified and designated as VtgAa, VtgAb, and VtgC, which confirmed the multiplicity of the vtg gene in turbot and showed that it follows a "three vtg model". The expression of these three vtg genes in the female liver was far higher than that in other tissues, including the ovary. The expression of all three vtg genes was extremely low before vitellogenesis, and then increased and was maintained at a high level until the degradation stage, which was in accordance with changes in the concentration of estradiol-17 beta (E-2) and the gonadosomatic index. Compared with the liver, the ovary had a higher E-2 level and lower vtg expression, suggesting that some other factors limit high vtg expression in the ovary of turbot. Transcripts of vtgAb and the Yps derived from them were both detected in oogonia and primary oocytes, which showed that these might possess the ability to perform autosynthesis of yolk. These findings add to our understanding of the reproductive physiology of Vtg synthesis in turbot. |
| 关键词 | Autosynthesis Estradiol-17 beta Turbot (Scophthalmus maximus) Vitellogenin Yolk protein |
| DOI | 10.1016/j.cbpb.2018.08.002 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; China Postdoctoral Science Foundation[2016M592259] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS (2014) ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; improved Seeds Engineering Project of Shandong Province (2014-2017) ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; China Postdoctoral Science Foundation[2016M592259] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS (2014) ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; improved Seeds Engineering Project of Shandong Province (2014-2017) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Zoology |
| WOS类目 | Biochemistry & Molecular Biology ; Zoology |
| WOS记录号 | WOS:000447114400007 |
| 出版者 | ELSEVIER SCIENCE INC |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160461 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua; Li, Jun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Xue, Rui,Wang, Xueying,Xu, Shihong,et al. Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2018,226:53-63. |
| APA | Xue, Rui.,Wang, Xueying.,Xu, Shihong.,Liu, Yifan.,Feng, Chengcheng.,...&Li, Jun.(2018).Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,226,53-63. |
| MLA | Xue, Rui,et al."Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 226(2018):53-63. |
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| Expression profile a（14470KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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