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Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge
Zhang, Jiquan1,2,3; Liu, Yujie1; Li, Yanyan1; Su, Naike1; Zhou, Yaru1; Xiang, Jianhai2,3; Sun, Yuying1
2018-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume82Pages:378-385
Corresponding AuthorSun, Yuying(sunyuying125@163.com)
AbstractThe gC1qR is a ubiquitously expressed cell protein that interacts with the globular heads of C1q (gC1q) and many other ligands. In this study, one gC1qR homolog gene was obtained from Exopalaemon carinicauda and named EcgC1qR. The complete nucleotide sequence of EcgC1qR contained a 774 bp open reading frame (ORF) encoding EcgC1qR precursor of 257 amino acids. The deduced amino acid sequence of EcgC1qR revealed a 55- amino-acid-long mitochondrial targeting sequence at the N-terminal and a mitochondrial acidic matrix protein of 33 kDa (MAM33) domain. The genomic organization of EcgC1qR gene showed that EcgC1qR gene contained five exons and four introns. EcgC1qR could express in all of the detected tissues and its expression was much higher in hepatopancreas and hemocytes. The expression of EcgC1qR in the hepatopancreas of prawns challenged with Vibrio parahaemolyticus and Aeromonas hydrophila changed in a time-dependent manner. The expression of EcgC1qR in prawns challenged with V. parahaemolyticus was up-regulated at 6h (p < 0.05), and significantly up-regulated at 12 h and 24 h (p < 0.01), and then returned to the control levels at 48 h post-challenge (p > 0.05). At the same time, the expression in Aeromonas-challenged group was significantly up-regulated at 6, 12 and 24 h. The recombinant EcgC1qR could inhibit the growth of two tested bacteria. In addition, we successfully deleted EcgC1qR gene through CRISPR/Cas9 technology and it was the first time to obtain the mutant of gC1qR homolog gene in crustacean. It's a great progress to study the biological function of gC1qR in crustacean in future.
KeywordExopalaemon carinicauda Globular heads of C1q CRISPR/Cas9 Knock-out
DOI10.1016/j.fsi.2018.08.046
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31872613] ; National Natural Science Foundation of China[41876196] ; National Natural Science Foundation of China[31772885]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000447571600044
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160437
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Yuying
Affiliation1.Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Jiquan,Liu, Yujie,Li, Yanyan,et al. Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2018,82:378-385.
APA Zhang, Jiquan.,Liu, Yujie.,Li, Yanyan.,Su, Naike.,Zhou, Yaru.,...&Sun, Yuying.(2018).Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge.FISH & SHELLFISH IMMUNOLOGY,82,378-385.
MLA Zhang, Jiquan,et al."Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge".FISH & SHELLFISH IMMUNOLOGY 82(2018):378-385.
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