Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain

doi:10.1111/j.1472-765X.2009.02761.x

IOCAS-IR > 实验海洋生物学重点实验室

	Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain
	Hu, Y-H.1; Dang, W.1,2; Liu, C-S.1,2; Sun, L.1; Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2010
发表期刊	LETTERS IN APPLIED MICROBIOLOGY
ISSN	0266-8254
卷号	50 期号:1 页码:97-103
文章类型	Article
摘要	Aim:; Aim: To investigate the effect of copper on the virulence of Edwardsiella tarda. Methods and Results: The pathogenic Edw. tarda strain TX5 was cultured under copper-stressed conditions and examined for any potential alteration in capacities that are associated with pathogenicity. The results showed that compared to untreated TX5, Cu-treated TX5 exhibits reduced planktonic and biofilm growth, an impaired ability to adhere to host mucus, modulation of host immune response, and dissemination in host blood and liver. Consistent with these observations, the overall bacterial virulence of Cu-treated TX5 is significantly attenuated. SDS-PAGE analyses of whole cell protein production showed that Cu-treated TX5 differs from the untreated TX5 in its production of at least one protein. Quantitative real time reverse transcriptase PCR analyses showed that copper treatment decreased the expression of virulence-associated genes encoding components of the type III and type VI secretion systems, the Eth haemolysin system, and the LuxS/AI-2 quorum-sensing system. Conclusions: Prolonged exposure to copper has multiple effects on TX5 and results in significant attenuation of bacterial virulence. Significance and Impact of the Study: The results of this study demonstrate that copper treatment has a broad and profound effect on the virulence-associated capacities of TX5, which is exerted at least in part at the transcription level. These findings provide new insights to the antimicrobial mechanism of copper.
关键词	Copper Edwardsiella Tarda Luxs Virulence
学科领域	Biotechnology & Applied Microbiology ; Microbiology
DOI	10.1111/j.1472-765X.2009.02761.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000272582600016
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1603
专题	实验海洋生物学重点实验室
通讯作者	Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Y-H.,Dang, W.,Liu, C-S.,et al. Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain[J]. LETTERS IN APPLIED MICROBIOLOGY,2010,50(1):97-103.
APA	Hu, Y-H.,Dang, W.,Liu, C-S.,Sun, L.,&Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain.LETTERS IN APPLIED MICROBIOLOGY,50(1),97-103.
MLA	Hu, Y-H.,et al."Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain".LETTERS IN APPLIED MICROBIOLOGY 50.1(2010):97-103.

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