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High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta)
Wang, Xiuliang1,2,4; Chen, Zhihang1,2,3; Li, Qiuying1,2,3; Zhang, Jie1,2,4; Liu, Shun1,2,3; Duan, Delin1,2,4
2018-09-11
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume8Pages:10
Corresponding AuthorDuan, Delin(dlduan@qdio.ac.cn)
AbstractSaccharina japonica is one of the most important marine crops in China, Japan and Korea. Candidate genes associated with blade length and blade width have not yet been reported. Here, based on SLAF-seq, the 7627 resulting SNP loci were selected for genetic linkage mapping to 31 linkage groups with an average spacing of 0.69 cM, and QTL analyses were performed to map the blade length and blade width phenotypes of S. japonica. In total, 12 QTLs contributing to blade length and 10 to width were detected. Some QTL intervals were detected for both blade length and width. Additive alleles for increasing blade length and width in S. japonica came from both parents. After the QTL interval regions were comparatively mapped to the current reference genome of S. japonica (MEHQ00000000), 14 Tic20 (translocon on the inner envelope membrane of chloroplast) genes and three peptidase genes were identified. RT-qPCR analysis showed that the transcription levels of four Tic20 genes were different not only in the two parent sporophytes but also at different cultivation times within one parent. The SNP markers closely associated with blade length and width could be used to improve the selection efficiency of S. japonica breeding.
DOI10.1038/s41598-018-32015-y
Indexed BySCI
Language英语
Funding ProjectNSFC[31772848] ; NSFC[31272660] ; Key Lab of Marine Bioactive Substance and Modern Analytical Technique, SOA ; National Key Technology Research and Development Program[2013BAB01B01] ; Ocean Public Welfare Scientific Research Project[201405040] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Shandong Key Sci-Technology Research Project[2016ZDJS06B2]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000444278400025
PublisherNATURE PUBLISHING GROUP
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160260
Collection实验海洋生物学重点实验室
Corresponding AuthorDuan, Delin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xiuliang,Chen, Zhihang,Li, Qiuying,et al. High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta)[J]. SCIENTIFIC REPORTS,2018,8:10.
APA Wang, Xiuliang,Chen, Zhihang,Li, Qiuying,Zhang, Jie,Liu, Shun,&Duan, Delin.(2018).High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta).SCIENTIFIC REPORTS,8,10.
MLA Wang, Xiuliang,et al."High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta)".SCIENTIFIC REPORTS 8(2018):10.
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