IOCAS-IR  > 实验海洋生物学重点实验室
Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai
Jia, Yanglei1,2,3,4; Liu, Xiao1,2,3
2018-09-07
Source PublicationFRONTIERS IN PHYSIOLOGY
ISSN1664-042X
Volume9Pages:15
AbstractNa+/K+-ATPase (NKA) belongs to the P-type ATPase family, whose members are located in the cell membrane and are distributed in diverse tissues and cells. The main function of the NKA is to regulate osmotic pressure. To better understand the role of NKA in osmoregulation, we first cloned and characterized the full-length cDNAs of NKA alpha subunit and beta subunit from Pacific abalone Haliotis discus hannai in the current study. The predicted protein sequence of the NKA a subunit, as the catalytic subunit, was well conserved. In contrast, the protein sequence of the beta subunit had low similarity with those of other species. Phylogenetic analysis revealed that both the alpha and beta subunits of the NKA protein of Pacific abalone were clustered with those of the Gastropoda. Then, the relationship between salinity changes and the NKA was investigated. Sudden salinity changes (with low-salinity seawater (LSW) or high-salinity seawater (HSW)) led to clear changes in ion concentration (Na+ and K+) in hemolymph; however, the relative stability of ion concentrations in tissue revealed that Pacific abalone has a strong osmotic pressure regulation ability when faced with these salinity changes. Meanwhile, the expression and activity of the NKA was significantly decreased (in LSW group) or increased (in HSW group) during the ion concentration re-establishing stages, which was consistent with the coordinated regulation of ion concentration in hemolymph. Moreover, a positive correlation between cyclic adenosine monophosphate (cAMP) concentrations and NKA mRNA expression (NKA activity) was observed in mantle and gill. Therefore, the sudden salinity changes may affect NKA transcription activation, translation and enzyme activity via a cAMP-mediated pathway.
Keywordsalinity osmoregulation ion transportation Na+/K+-ATPase cAMP
DOI10.3389/fphys.2018.01244
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41676152] ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province[2017LZGC009]
WOS Research AreaPhysiology
WOS SubjectPhysiology
WOS IDWOS:000444061000001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/160242
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Xiao
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Yanglei,Liu, Xiao. Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai[J]. FRONTIERS IN PHYSIOLOGY,2018,9:15.
APA Jia, Yanglei,&Liu, Xiao.(2018).Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai.FRONTIERS IN PHYSIOLOGY,9,15.
MLA Jia, Yanglei,et al."Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai".FRONTIERS IN PHYSIOLOGY 9(2018):15.
Files in This Item:
File Name/Size DocType Version Access License
Expression of Na+K+-(7116KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jia, Yanglei]'s Articles
[Liu, Xiao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jia, Yanglei]'s Articles
[Liu, Xiao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jia, Yanglei]'s Articles
[Liu, Xiao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Expression of Na+K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.