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Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai
Jia, Yanglei1,2,3,4; Liu, Xiao1,2,3
2018-09-07
发表期刊FRONTIERS IN PHYSIOLOGY
ISSN1664-042X
卷号9页码:15
摘要Na+/K+-ATPase (NKA) belongs to the P-type ATPase family, whose members are located in the cell membrane and are distributed in diverse tissues and cells. The main function of the NKA is to regulate osmotic pressure. To better understand the role of NKA in osmoregulation, we first cloned and characterized the full-length cDNAs of NKA alpha subunit and beta subunit from Pacific abalone Haliotis discus hannai in the current study. The predicted protein sequence of the NKA a subunit, as the catalytic subunit, was well conserved. In contrast, the protein sequence of the beta subunit had low similarity with those of other species. Phylogenetic analysis revealed that both the alpha and beta subunits of the NKA protein of Pacific abalone were clustered with those of the Gastropoda. Then, the relationship between salinity changes and the NKA was investigated. Sudden salinity changes (with low-salinity seawater (LSW) or high-salinity seawater (HSW)) led to clear changes in ion concentration (Na+ and K+) in hemolymph; however, the relative stability of ion concentrations in tissue revealed that Pacific abalone has a strong osmotic pressure regulation ability when faced with these salinity changes. Meanwhile, the expression and activity of the NKA was significantly decreased (in LSW group) or increased (in HSW group) during the ion concentration re-establishing stages, which was consistent with the coordinated regulation of ion concentration in hemolymph. Moreover, a positive correlation between cyclic adenosine monophosphate (cAMP) concentrations and NKA mRNA expression (NKA activity) was observed in mantle and gill. Therefore, the sudden salinity changes may affect NKA transcription activation, translation and enzyme activity via a cAMP-mediated pathway.
关键词salinity osmoregulation ion transportation Na+/K+-ATPase cAMP
DOI10.3389/fphys.2018.01244
收录类别SCI
语种英语
资助项目Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province[2017LZGC009] ; National Natural Science Foundation of China[41676152] ; National Natural Science Foundation of China[41676152] ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province[2017LZGC009]
WOS研究方向Physiology
WOS类目Physiology
WOS记录号WOS:000444061000001
出版者FRONTIERS MEDIA SA
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/160242
专题实验海洋生物学重点实验室
通讯作者Liu, Xiao
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
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Jia, Yanglei,Liu, Xiao. Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai[J]. FRONTIERS IN PHYSIOLOGY,2018,9:15.
APA Jia, Yanglei,&Liu, Xiao.(2018).Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai.FRONTIERS IN PHYSIOLOGY,9,15.
MLA Jia, Yanglei,et al."Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai".FRONTIERS IN PHYSIOLOGY 9(2018):15.
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