IOCAS-IR  > 实验海洋生物学重点实验室
A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells
Geng, Lihua1,2,3,4; Hu, Weicheng5; Liu, Yingjuan1,2,3; Wang, Jing1,2,3,4; Zhang, Quanbin1,2,3,4
2018-12-01
Source PublicationCARBOHYDRATE POLYMERS
ISSN0144-8617
Volume201Pages:557-565
Corresponding AuthorZhang, Quanbin(qbzhang@qdio.ac.cn)
AbstractA heteropolysaccharide (SHP) with a strong immunomodulatory effect on RAW 264.7 cells was prepared from Saccharina japonica. Chemical analysis demonstrated that SHP was primarily composed of mannose, glucuronic acid, glucose, fucose, galactose, xylose and rahmnose with a molar ratio of 1.00:0.85:0.84:0.58:0.30:0.37:0.15. ESI-MS showed that depolymerized SHP produced oligo-glucuronan, oligo-glucuronomannan, sulfated fuco-oligosaccharides and other hetero-oligosaccharides. The in vitro immunomodulatory results showed that SHP could increase NO production and up-regulate the expression of many immune effectors, including iNOS, COX-2 and TNF-alpha, displaying an apparent immune enhancement activities. Western blot analysis proved that SHP activated the expression levels of many key components involved in NF-kappa B, MAPK and Akt signaling pathways. Our results together indicated that SHP has the potential to be developed as a novel immunomodulator for activating immune system.
KeywordSaccharina japonica Polysaccharide Immunomodulatory activity Macrophage
DOI10.1016/j.carbpol.2018.08.096
Indexed BySCI
Language英语
Funding ProjectFujian STS project of Chinese Academy of Sciences ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Key Research and Development Project of Shandong Province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190] ; K.C. Wong Education Foundation, CAS
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000445030100060
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160230
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Quanbin
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Huaian 223300, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Geng, Lihua,Hu, Weicheng,Liu, Yingjuan,et al. A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells[J]. CARBOHYDRATE POLYMERS,2018,201:557-565.
APA Geng, Lihua,Hu, Weicheng,Liu, Yingjuan,Wang, Jing,&Zhang, Quanbin.(2018).A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells.CARBOHYDRATE POLYMERS,201,557-565.
MLA Geng, Lihua,et al."A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells".CARBOHYDRATE POLYMERS 201(2018):557-565.
Files in This Item:
File Name/Size DocType Version Access License
A heteropolysacchari(2011KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Geng, Lihua]'s Articles
[Hu, Weicheng]'s Articles
[Liu, Yingjuan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Geng, Lihua]'s Articles
[Hu, Weicheng]'s Articles
[Liu, Yingjuan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Geng, Lihua]'s Articles
[Hu, Weicheng]'s Articles
[Liu, Yingjuan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.