IOCAS-IR
Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex
Cheng, Jiao1,2,3; Zhang, Nan1; Sha, Zhong-li1,2,3
2018-10-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume45Issue:5Pages:1499-1505
Corresponding AuthorSha, Zhong-li(shazl@qdio.ac.cn)
AbstractThe Japanese mantis shrimp Oratosquilla oratoria is one of the most dominant stomatopod species found in coastal waters of the northwestern Pacific. We previously reported cryptic speciation and hybridization in this taxon by integrating mitochondrial and nuclear evidence. The present study aims at developing potential useful microsatellite markers for the O. oratoria complex through transcriptome sequencing, with a view to reveal the occurrence of hybridization. Of the 100 tested microsatellites, 55 were experimentally validated. 24 of these microsatellites were transferable across different species of the O. oratoria complex and showed polymorphic among individuals. The average number of alleles, observed and expected heterozygosity per locus was 6.125, 0.446 and 0.577 for the temperate species, and 6.083, 0.444 and 0.578 for the subtropical and tropical species. We also explore genetic differentiation and hybridization between O. oratoria cryptic species using these 24 microsatellite loci. The pairwise F (ST) values and phylogenetic tree indicated a strong genetic differentiation between the two cryptic species. In addition, Bayesian analysis provided evidence for the presence of hybridization between the O. oratoria complex. These markers provide valuable genomic resources for exploring introgressive hybridization and expanding understanding of evolution in the O. oratoria complex.
KeywordOratosquilla oratoria Transcriptome Microsatellite Hybridization
DOI10.1007/s11033-018-4208-6
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31401955] ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSW-DQC036]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000444752900079
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160227
Collection中国科学院海洋研究所
Corresponding AuthorSha, Zhong-li
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Cheng, Jiao,Zhang, Nan,Sha, Zhong-li. Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex[J]. MOLECULAR BIOLOGY REPORTS,2018,45(5):1499-1505.
APA Cheng, Jiao,Zhang, Nan,&Sha, Zhong-li.(2018).Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex.MOLECULAR BIOLOGY REPORTS,45(5),1499-1505.
MLA Cheng, Jiao,et al."Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex".MOLECULAR BIOLOGY REPORTS 45.5(2018):1499-1505.
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