Knowledge Management System Of Institute of Oceanology, Chinese Academy of Sciences
| Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex | |
Cheng, Jiao1,2,3; Zhang, Nan1; Sha, Zhong-li1,2,3
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| 2018-10-01 | |
| 发表期刊 | MOLECULAR BIOLOGY REPORTS
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| ISSN | 0301-4851 |
| 卷号 | 45期号:5页码:1499-1505 |
| 通讯作者 | Sha, Zhong-li(shazl@qdio.ac.cn) |
| 摘要 | The Japanese mantis shrimp Oratosquilla oratoria is one of the most dominant stomatopod species found in coastal waters of the northwestern Pacific. We previously reported cryptic speciation and hybridization in this taxon by integrating mitochondrial and nuclear evidence. The present study aims at developing potential useful microsatellite markers for the O. oratoria complex through transcriptome sequencing, with a view to reveal the occurrence of hybridization. Of the 100 tested microsatellites, 55 were experimentally validated. 24 of these microsatellites were transferable across different species of the O. oratoria complex and showed polymorphic among individuals. The average number of alleles, observed and expected heterozygosity per locus was 6.125, 0.446 and 0.577 for the temperate species, and 6.083, 0.444 and 0.578 for the subtropical and tropical species. We also explore genetic differentiation and hybridization between O. oratoria cryptic species using these 24 microsatellite loci. The pairwise F (ST) values and phylogenetic tree indicated a strong genetic differentiation between the two cryptic species. In addition, Bayesian analysis provided evidence for the presence of hybridization between the O. oratoria complex. These markers provide valuable genomic resources for exploring introgressive hybridization and expanding understanding of evolution in the O. oratoria complex. |
| 关键词 | Oratosquilla oratoria Transcriptome Microsatellite Hybridization |
| DOI | 10.1007/s11033-018-4208-6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31401955] ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSW-DQC036] ; National Natural Science Foundation of China[31401955] ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSW-DQC036] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000444752900079 |
| 出版者 | SPRINGER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160227 |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Sha, Zhong-li |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, Jiao,Zhang, Nan,Sha, Zhong-li. Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex[J]. MOLECULAR BIOLOGY REPORTS,2018,45(5):1499-1505. |
| APA | Cheng, Jiao,Zhang, Nan,&Sha, Zhong-li.(2018).Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex.MOLECULAR BIOLOGY REPORTS,45(5),1499-1505. |
| MLA | Cheng, Jiao,et al."Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex".MOLECULAR BIOLOGY REPORTS 45.5(2018):1499-1505. |
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| Isolation and charac（1835KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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