IOCAS-IR  > 海洋环境腐蚀与与生物污损重点实验室
DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex
Zheng, Laibao1,2,3,4; Qi, Peng1,2,4; Zhang, Dun1,2,4
2018-12-10
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume276Pages:42-47
Corresponding AuthorQi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
AbstractThe rapid and sensitive detection of bacteria is of great importance for the field of food safety, water quality and clinical diagnosis. Herein, we present a novel DNA-templated fluorescent silver nanoclusters (DNA-AgNCs) based sensing system integrated with magnetic nanoparticles (MNP)-DNAzyme-AChE complex for detection of pathogenic bacterial using Escherichia coli (E. coli) as a target bacteria. The platform takes advantage of a DNA-AgNCs based enzyme-responsive fluorescent signal element and a DNAzyme recognition element, providing an ultrasensitive detection limit of 60 cfu mL(-1), and good specificity to the target bacteria. Moreover, the platform could be developed into a potentially versatile system by simply altering corresponding DNAzyme as the objects recognition probes.
KeywordDNA-template silver nanocluster DNAzyme DNA-enzyme conjugate Bacterial detection
DOI10.1016/j.snb.2018.08.078
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; Science & Technology Basic Research Program of Qingdao[16-5-1-19-jch] ; Qingdao National Laboratory for Marine Science and Technology[017ASTCP-ES02]
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000444637600006
PublisherELSEVIER SCIENCE SA
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160206
Collection海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorQi, Peng; Zhang, Dun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zheng, Laibao,Qi, Peng,Zhang, Dun. DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,276:42-47.
APA Zheng, Laibao,Qi, Peng,&Zhang, Dun.(2018).DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex.SENSORS AND ACTUATORS B-CHEMICAL,276,42-47.
MLA Zheng, Laibao,et al."DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex".SENSORS AND ACTUATORS B-CHEMICAL 276(2018):42-47.
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