Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex | |
Zheng, Laibao1,2,3,4 ; Qi, Peng1,2,4; Zhang, Dun1,2,4
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| 2018-12-10 | |
| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
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| ISSN | 0925-4005 |
| 卷号 | 276页码:42-47 |
| 通讯作者 | Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) |
| 摘要 | The rapid and sensitive detection of bacteria is of great importance for the field of food safety, water quality and clinical diagnosis. Herein, we present a novel DNA-templated fluorescent silver nanoclusters (DNA-AgNCs) based sensing system integrated with magnetic nanoparticles (MNP)-DNAzyme-AChE complex for detection of pathogenic bacterial using Escherichia coli (E. coli) as a target bacteria. The platform takes advantage of a DNA-AgNCs based enzyme-responsive fluorescent signal element and a DNAzyme recognition element, providing an ultrasensitive detection limit of 60 cfu mL(-1), and good specificity to the target bacteria. Moreover, the platform could be developed into a potentially versatile system by simply altering corresponding DNAzyme as the objects recognition probes. |
| 关键词 | DNA-template silver nanocluster DNAzyme DNA-enzyme conjugate Bacterial detection |
| DOI | 10.1016/j.snb.2018.08.078 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; Science & Technology Basic Research Program of Qingdao[16-5-1-19-jch] ; Qingdao National Laboratory for Marine Science and Technology[017ASTCP-ES02] ; National Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; Science & Technology Basic Research Program of Qingdao[16-5-1-19-jch] ; Qingdao National Laboratory for Marine Science and Technology[017ASTCP-ES02] |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| WOS记录号 | WOS:000444637600006 |
| 出版者 | ELSEVIER SCIENCE SA |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160206 |
| 专题 | 海洋环境腐蚀与生物污损重点实验室 |
| 通讯作者 | Qi, Peng; Zhang, Dun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zheng, Laibao,Qi, Peng,Zhang, Dun. DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,276:42-47. |
| APA | Zheng, Laibao,Qi, Peng,&Zhang, Dun.(2018).DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex.SENSORS AND ACTUATORS B-CHEMICAL,276,42-47. |
| MLA | Zheng, Laibao,et al."DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex".SENSORS AND ACTUATORS B-CHEMICAL 276(2018):42-47. |
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| DNA-templated fluore（908KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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