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Spermatogonial stem cells differentiation and testicular lobules formation in a seasonal breeding teleost: The evidence from the heat-induced masculinization of genetically female Japanese flounder (Paralichthys olivaceus)
Yang, Yang1,2,3; Liu, Qinghua1,2; Ma, Daoyuan1,2; Xiao, Yongshuang1,2; Xu, Shihong1,2; Wang, Xueying1,2; Song, Zongcheng4; You, Feng1,2; Li, Jun1,2
2018-10-15
Source PublicationTHERIOGENOLOGY
ISSN0093-691X
Volume120Pages:68-78
AbstractGerm cells undergo a series of cellular changes including differentiation, mitosis, meiosis and maturation, and eventually develop into a large number of functional gametes. The available data regarding teleost gametogenesis in seasonal batch spawners is limited. In this study, we investigated spermatogenesis with special attention on spermatogonia differentiation using heat-induced masculine juveniles of genetically female Japanese flounder. Meanwhile, the Nanos2 expression had been detected by immunohistochemistry for analysis of spermatogonial stem cells (SSCs) distribution. Spermatogonia began mitosis at 35 dph, and basement membrane firstly appeared and gradually surrounded and separated the spermatogonia (type A) into single and paired status. At this period, the spermatogonia continuously maintained mitotic proliferation. As a result, the number of spermatogonia including isolated and clusters (2-8 spermatogonia) significantly increased in the presumptive testes. From 85 dph to 120 dph, with the mitosis of spermatogonia, germline acinar-clusters formed. In the clusters, type A spermatogonia differentiated into type B, and multi-spermatogonia surrounded by several sertoli cell formed cysts , which represented the formation of lobular precursors. After that, type B spermatogonia began meiosis, which indicated the initiation of spermatogenesis. In adult testes, most type A spermatogonia distributed in the peripheral region and a few clung to a basement membrane in the internal germinal epithelium. Various spermatogenic cysts with germ cells in different developmental stages existed in a testicular lobules, moreover the germ cells in earlier stages resided in the distal termini, and the advanced stages were adjacent to the spermatic duct of testes. Therefore, the testes of Japanese flounder belonged to an intermediated distribution of SSCs, which might contribute greatly to multiple spermiation during breeding season. These findings would improve the understanding the mechanisms of SSCs differentiation and testicular development, and may be of great value in future studies of the spermatogenesis regulation. (C) 2018 Elsevier Inc. All rights reserved.
KeywordSpermatogenesis Basement membrane Testicular lobules Spermatogonia Nanos2
DOI10.1016/j.theriogenology.2018.07.041
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Postdoctoral Science Foundation[2016M592259] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; Modern Agro-Industry Technology Research System[nycytx-50] ; improved Seeds Engineering Project of Shandong Province (2014-2017)
WOS Research AreaReproductive Biology ; Veterinary Sciences
WOS SubjectReproductive Biology ; Veterinary Sciences
WOS IDWOS:000444782300010
PublisherELSEVIER SCIENCE INC
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/160160
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Qinghua; Li, Jun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Weihai Shenghang Aquat Prod Sci & Technol Co, Weihai 264200, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Yang,Liu, Qinghua,Ma, Daoyuan,et al. Spermatogonial stem cells differentiation and testicular lobules formation in a seasonal breeding teleost: The evidence from the heat-induced masculinization of genetically female Japanese flounder (Paralichthys olivaceus)[J]. THERIOGENOLOGY,2018,120:68-78.
APA Yang, Yang.,Liu, Qinghua.,Ma, Daoyuan.,Xiao, Yongshuang.,Xu, Shihong.,...&Li, Jun.(2018).Spermatogonial stem cells differentiation and testicular lobules formation in a seasonal breeding teleost: The evidence from the heat-induced masculinization of genetically female Japanese flounder (Paralichthys olivaceus).THERIOGENOLOGY,120,68-78.
MLA Yang, Yang,et al."Spermatogonial stem cells differentiation and testicular lobules formation in a seasonal breeding teleost: The evidence from the heat-induced masculinization of genetically female Japanese flounder (Paralichthys olivaceus)".THERIOGENOLOGY 120(2018):68-78.
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