A potent anti-oxidant property: fluorescent recombinant alpha-phycocyanin of Spirulina

doi:10.1111/j.1365-2672.2008.04051.x

IOCAS-IR > 实验海洋生物学重点实验室

	A potent anti-oxidant property: fluorescent recombinant alpha-phycocyanin of Spirulina
	Guan, X. Y.1; Zhang, W. J.2,3; Zhang, X. W.2,3; Li, Y. X.2,3; Wang, J. F.2,3; Lin, H. Z.2,3; Tang, X. X.1; Qin, S.2; Tang, XX, Ocean Univ China, Coll Marine Life Sci, Hong Dao Rd 5, Qingdao 266003, Peoples R China
	2009-04-01
发表期刊	JOURNAL OF APPLIED MICROBIOLOGY
ISSN	1364-5072
卷号	106 期号:4 页码:1093-1100
文章类型	Article
摘要	To express and product a fluorescent antioxidant holo-alpha-phycocyanin (PC) of Spirulina platensis (Sp) with His-tag (rHHPC; recombinant holo-alpha-phycocyaninof Spirulina platensis with His-tag) in 5-l bench scale.; To express and product a fluorescent antioxidant holo-alpha-phycocyanin (PC) of Spirulina platensis (Sp) with His-tag (rHHPC; recombinant holo-alpha-phycocyaninof Spirulina platensis with His-tag) in 5-l bench scale. A vector harbouring two cassettes was constructed: cpcA along with cpcE-cpcF in one cassette; ho1-pcyA in the other cassette. Lyases CpcE/F of Synechocystis sp. PCC6803 (S6) could catalyse the 82 site Cys in apo-alpha-PC of Sp linking with bilin chromophores, and rHHPC was biosynthesized in Escherichia coli BL21. The constant feeding mode was adopted, and transformant reached the biomass of rHHPC up to 0.55 g l(-1) broth in 5-litre bench scale. rHHPC was purified by Ni2+ affinity column conveniently. The absorbance and the fluorescence emission spectra of rHHPC had lambda(max) at 621 and 650 nm, respectively. The IC50 values of rHHPC were 277.5 +/- 25.8 mu g ml(-1) against hydroxyl radicals and 20.8 +/- 2.2 mu g ml(-1) against peroxyl radicals. Combinational biosynthesis of rHHPC was feasible, and the constant feeding mode was adopted to produce good yields of rHHPC. Fluorescent rHHPC with several unique qualitative and quantitative features was effective on scavenging hydroxyl and peroxyl radicals. A potent antioxidant rHHPC was co-expressed, produced and characterized for nutritional and pharmacological values, which would help to develop phycobiliproteins' applications in their fluorescent and biological activities.
关键词	Antioxidant Phycocyanin Rhhpc Spirulina Platensis
学科领域	Biotechnology & Applied Microbiology ; Microbiology
DOI	10.1111/j.1365-2672.2008.04051.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000264022300004
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1599
专题	实验海洋生物学重点实验室
通讯作者	Tang, XX, Ocean Univ China, Coll Marine Life Sci, Hong Dao Rd 5, Qingdao 266003, Peoples R China
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Guan, X. Y.,Zhang, W. J.,Zhang, X. W.,et al. A potent anti-oxidant property: fluorescent recombinant alpha-phycocyanin of Spirulina[J]. JOURNAL OF APPLIED MICROBIOLOGY,2009,106(4):1093-1100.
APA	Guan, X. Y..,Zhang, W. J..,Zhang, X. W..,Li, Y. X..,Wang, J. F..,...&Tang, XX, Ocean Univ China, Coll Marine Life Sci, Hong Dao Rd 5, Qingdao 266003, Peoples R China.(2009).A potent anti-oxidant property: fluorescent recombinant alpha-phycocyanin of Spirulina.JOURNAL OF APPLIED MICROBIOLOGY,106(4),1093-1100.
MLA	Guan, X. Y.,et al."A potent anti-oxidant property: fluorescent recombinant alpha-phycocyanin of Spirulina".JOURNAL OF APPLIED MICROBIOLOGY 106.4(2009):1093-1100.

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