Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei) | |
Liu, Xinwei1,2,3; Wang, Mengqiang1,2 ; Sun, Guoqiong1,2; Wang, Baojie1,2; Jiang, Keyong1,2; Shao, Jianchun1,2,3; Qi, Cancan1,2,3; Zhao, Wei4; Han, Siyin1,2,3; Liu, Mei1,2; Wang, Lei1,2
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| 2018-10-01 | |
| 发表期刊 | AQUACULTURE NUTRITION
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| ISSN | 1353-5773 |
| 卷号 | 24期号:5页码:1509-1520 |
| 通讯作者 | Liu, Mei(liumei@qdio.ac.cn) ; Wang, Lei(wanglei@qdio.ac.cn) |
| 摘要 | Mechanistic target of rapamycin (mTOR) is a central regulator of cell growth and metabolism including autophagy. The mTORC1 signalling pathway is a major regulator of autophagy in response to nutrient levels. The drug rapamycin induces autophagy in various cell types and species by specifically inhibiting the activity of mTOR. To identify the genes and correlative regulatory pathways involved in cell metabolism and autophagy of Litopenaeus vannamei, we analysed the transcriptome of shrimps treated with rapamycin. We found that the expression of some genes related to autophagy was significantly changed. To further explore the autophagy regulatory mechanism, the concentration and phosphorylation of related proteins were analysed by Western blot. We found that treatment with the rapamycin can depress phosphorylating of ATG1 and promote dephosphorylating of ATG13. There are some heat-shock protein genes and genes involving apoptosis, function of lysosome and feedback regulation of mTOR signalling path differently expressed. The relationship between them and autophagy induced by inhibiting mTOR is also discussed. Evidences show the complicated positive and negative feedback loops controlling autophagy crustaceans are scarce. Our study verifies and contributes to the current understanding of autophagy induced by mTOR in crustacean, providing an abundant source for the identification of novel genes. |
| 关键词 | autophagy Litopenaeus vannamei mTOR rapamycin transcriptome |
| DOI | 10.1111/anu.12688 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[41406151] ; Science and Technology Major Project of Shandong Province[2015ZDZX05002] ; Chinese Academy of Sciences STS Major Deployment Project[KFZD-SW-106] ; National Natural Science Foundation of China[41406151] ; Science and Technology Major Project of Shandong Province[2015ZDZX05002] ; Chinese Academy of Sciences STS Major Deployment Project[KFZD-SW-106] |
| WOS研究方向 | Fisheries |
| WOS类目 | Fisheries |
| WOS记录号 | WOS:000443545000014 |
| 出版者 | WILEY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/159989 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Mei; Wang, Lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Tianjin Agr Univ, Coll Fishery Sci, Tianjin Key Lab Aquaculture, Tianjin, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Xinwei,Wang, Mengqiang,Sun, Guoqiong,et al. Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)[J]. AQUACULTURE NUTRITION,2018,24(5):1509-1520. |
| APA | Liu, Xinwei.,Wang, Mengqiang.,Sun, Guoqiong.,Wang, Baojie.,Jiang, Keyong.,...&Wang, Lei.(2018).Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei).AQUACULTURE NUTRITION,24(5),1509-1520. |
| MLA | Liu, Xinwei,et al."Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)".AQUACULTURE NUTRITION 24.5(2018):1509-1520. |
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| Mechanistic target o（1148KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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