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A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)
Yang Yang1,2,3; Liu Qinghua1,2; Ma Daoyuan1,2; Song Zongcheng4; Li Jun1,2
2018-07-01
发表期刊JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
卷号36期号:4页码:1342-1348
通讯作者Liu Qinghua(qinghualiu@qdio.ac.cn) ; Li Jun(junli@qdio.ac.cn)
摘要Some germ cell marker genes, such as vasa, nanos, and dead end (dnd), have been identified in fish. Recently, lymphocyte antigen 75 (Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog (ly75) was cloned and its expression pattern in gonads was analyzed. The full-length cDNA of ly75 was 7 346 bp, with an open reading frame (ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 kDa. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.
关键词Ly75/CD205 Japanese flounder Paralichthys olivaceus gonads germ cell-specific marker
DOI10.1007/s00343-019-7390-z
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[31372514] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; National Natural Science Foundation of China[31372514] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060]
WOS研究方向Marine & Freshwater Biology ; Oceanography
WOS类目Limnology ; Oceanography
WOS记录号WOS:000441231100028
出版者SCIENCE PRESS
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/159941
专题实验海洋生物学重点实验室
通讯作者Liu Qinghua; Li Jun
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
第一作者单位实验海洋生物学重点实验室
通讯作者单位实验海洋生物学重点实验室
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GB/T 7714
Yang Yang,Liu Qinghua,Ma Daoyuan,et al. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1342-1348.
APA Yang Yang,Liu Qinghua,Ma Daoyuan,Song Zongcheng,&Li Jun.(2018).A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205).JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1342-1348.
MLA Yang Yang,et al."A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1342-1348.
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