IOCAS-IR  > 实验海洋生物学重点实验室
A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)
Yang Yang1,2,3; Liu Qinghua1,2; Ma Daoyuan1,2; Song Zongcheng4; Li Jun1,2
2018-07-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:4Pages:1342-1348
Corresponding AuthorLiu Qinghua(qinghualiu@qdio.ac.cn) ; Li Jun(junli@qdio.ac.cn)
AbstractSome germ cell marker genes, such as vasa, nanos, and dead end (dnd), have been identified in fish. Recently, lymphocyte antigen 75 (Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog (ly75) was cloned and its expression pattern in gonads was analyzed. The full-length cDNA of ly75 was 7 346 bp, with an open reading frame (ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 kDa. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.
KeywordLy75/CD205 Japanese flounder Paralichthys olivaceus gonads germ cell-specific marker
DOI10.1007/s00343-019-7390-z
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31372514] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000441231100028
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159941
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu Qinghua; Li Jun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang Yang,Liu Qinghua,Ma Daoyuan,et al. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1342-1348.
APA Yang Yang,Liu Qinghua,Ma Daoyuan,Song Zongcheng,&Li Jun.(2018).A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205).JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1342-1348.
MLA Yang Yang,et al."A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1342-1348.
Files in This Item:
File Name/Size DocType Version Access License
A potential germ cel(870KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang Yang]'s Articles
[Liu Qinghua]'s Articles
[Ma Daoyuan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang Yang]'s Articles
[Liu Qinghua]'s Articles
[Ma Daoyuan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang Yang]'s Articles
[Liu Qinghua]'s Articles
[Ma Daoyuan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus identification and characterization of lymphocyte antigen 75 (Ly75CD205).pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.