IOCAS-IR
Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas
Li, Chunyan1,2,3,5,6; Wang, Jinpeng1,2,3,5,6; Song, Kai1,3,5,6; Meng, Jie1,3,5,6; Xu, Fei1,3,5,6; Li, Li1,4,5,6; Zhang, Guofan1,3,5,6
2018-08-22
Source PublicationBMC GENOMICS
ISSN1471-2164
Volume19Pages:15
Corresponding AuthorLi, Li(lili@qdio.ac.cn) ; Zhang, Guofan(gfzhang@qdio.ac.cn)
AbstractBackground: Both growth and nutritional traits are important economic traits of Crassostrea gigas (C. gigas) in industry. But few work has been done to study the genetic architecture of nutritional traits of the oyster. In this study, we constructed a high-density genetic map of C. gigas to help assemble the genome sequence onto chromosomes, meanwhile explore the genetic basis for nutritional traits via quantitative trait loci (QTL) mapping. Results: The constructed genetic map contained 5024 evenly distributed markers, with an average marker interval of 0.68 cM, thus representing the densest genetic map produced for the oyster. According to the high collinearity between the consensus map and the oyster genome, 1574 scaffold (about 70%) of the genome sequence of C. gigas were successfully anchored to 10 linkage groups (LGs) of the consensus map. Using this high-qualified genetic map, we then conducted QTL analysis for growth and nutritional traits, the latter of which includes glycogen, amino acid (AA), and fatty acid (FA). Overall, 41 QTLs were detected for 17 traits. In addition, six candidate genes identified in the QTL interval showed significant correlation with the traits on transcriptional levels. These genes include growth-related genes AMY and BMP1, AA metabolism related genes PLSCR and GR, and FA metabolism regulation genes DYRK and ADAMTS. Conclusion: Using the constructed high-qualified linkage map, this study not only assembled nearly 70% of the oyster genome sequence onto chromosomes, but also identified valuable markers and candidate genes for growth and nutritional traits, especially for AA and FA that undergone few studies before. These findings will facilitate genome assembly and molecular breeding of important economic traits in C. gigas.
KeywordCrassostrea gigas Genetic map Collinearity Quantitative trait loci (QTL) mapping Growth Nutrition
DOI10.1186/s12864-018-4996-z
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31530079] ; National Natural Science Foundation of China[31572620] ; Strategic Priority Research Program of Western Pacific Ocean System: Structure, Dynamics and Consequences[XDA586 11020305] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49]
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000442532000005
PublisherBMC
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159887
Collection中国科学院海洋研究所
Corresponding AuthorLi, Li; Zhang, Guofan
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Chunyan,Wang, Jinpeng,Song, Kai,et al. Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas[J]. BMC GENOMICS,2018,19:15.
APA Li, Chunyan.,Wang, Jinpeng.,Song, Kai.,Meng, Jie.,Xu, Fei.,...&Zhang, Guofan.(2018).Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas.BMC GENOMICS,19,15.
MLA Li, Chunyan,et al."Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas".BMC GENOMICS 19(2018):15.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Chunyan]'s Articles
[Wang, Jinpeng]'s Articles
[Song, Kai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Chunyan]'s Articles
[Wang, Jinpeng]'s Articles
[Song, Kai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Chunyan]'s Articles
[Wang, Jinpeng]'s Articles
[Song, Kai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.