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Histo-blood group antigens in Crassostrea gigas and binding profiles with GII.4 Norovirus
Ma Liping1,2,3; Liu Hui2; Su Laijin2; Zhao Feng2; Zhou Deqing2; Duan Delin1,3
2018-07-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:4Pages:1383-1391
Corresponding AuthorZhou Deqing(zhoudq@ysfri.ac.cn) ; Duan Delin(dlduan@qdio.ac.cn)
AbstractNoroviruses (NoVs) are the main cause of viral gastroenteritis outbreaks worldwide, and oysters are the most common carriers of NoV contamination and transmission. NoVs bind specifically to oyster tissues through histo-blood group antigens (HBGAs), and this facilitates virus accumulation and increases virus persistence in oysters. To investigate the interaction of HBGAs in Pacific oysters with GII.4 NoV, we examined HBGAs with ELISAs and investigated binding patterns with oligosaccharide-binding assays using P particles as a model of five GII.4 NoV capsids. The HBGAs in the gut and gills exhibited polymorphisms. In the gut, type A was detected (100%), whereas type Leb (91.67%) and type A (61.11%) were both observed in the gills. Moreover, we found that seasonal NoV gastroenteritis outbreaks were not significantly associated with the specific HBGAs detected in the oyster gut and gills. In the gut, we found that strain-2006b and strain-96/96US bound to type A and H1 but only weakly bound to type Leb; in contrast, the Camberwell and Hunter strains exhibited weak binding to types H1 and Ley, and strain-Sakai exhibited no binding to any HBGA type. In the gills, strain-96/96US and strain-2006b bound to type Leb but only weakly bound to type H1; strains Camberwell, Hunter, and Sakai did not bind to oyster HBGAs. Assays for oligosaccharide binding to GII.4 NoV P particles showed that strain-95/96US and strain-2006b strongly bound to type A, B, H1, Leb, and Ley oligosaccharides, while strains Camberwell and Hunter showed weak binding ability to type H1 and Ley oligosaccharides and strain-Sakai showed weak binding ability to type Leb and Ley oligosaccharides. Our study presents new information and enhances understanding about the mechanism for NoV accumulation in oysters. Further studies of multiple NoV-tissue interactions might assist in identifying new or improved strategies for minimizing contamination, including HBGA-based attachment inhibition or depuration.
KeywordCrassostrea gigas norovirus histo-blood group antigen binding
DOI10.1007/s00343-018-7024-x
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31471663] ; Qingdao Postdoctoral Application Research Project
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000441231100032
PublisherSCIENCE PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159877
Collection实验海洋生物学重点实验室
Corresponding AuthorZhou Deqing; Duan Delin
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ma Liping,Liu Hui,Su Laijin,et al. Histo-blood group antigens in Crassostrea gigas and binding profiles with GII.4 Norovirus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1383-1391.
APA Ma Liping,Liu Hui,Su Laijin,Zhao Feng,Zhou Deqing,&Duan Delin.(2018).Histo-blood group antigens in Crassostrea gigas and binding profiles with GII.4 Norovirus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1383-1391.
MLA Ma Liping,et al."Histo-blood group antigens in Crassostrea gigas and binding profiles with GII.4 Norovirus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1383-1391.
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