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Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus
Zhao Chunyan1,2,3; Xu Shihong1,2; Feng Chengcheng1,2,3; Liu Yifan1,3; Yang Yang1,2,3; Wang Yanfeng1,3; Xiao Yongshuang1,3; Song Zongcheng4; Liu Qinghua1,3; Li Jun1,3
2018-07-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:4Pages:1360-1373
Corresponding AuthorLiu Qinghua(qinghualiu@qdio.ac.cn) ; Li Jun(junli@qdio.ac.cn)
AbstractTurbots (Schophthalmus maximus), one of the most important economic marine flatfish species, fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates, reproduction is regulated by the brain-pituitary-gonadal axis (BPG-axis), and gonadotropin releasing hormone (GnRH) is one of its key components. Therefore, to better understand the physiology of reproduction in the turbot, three of the genes encoding GnRH subtypes-sbGnRH, cGnRH - II and sGnRH - were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain; sbGnRH and sGnRH mRNAs were also detected in the gonads and pituitary gland, and sbGnRH expression was much higher than that of sGnRH, indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover, the brain expression patterns of sbGnRH and sGnRH mRNAs showed an increased trend during gonadal development, peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition, cGnRH - II mRNA expression showed no significant variations, suggesting that cGnRH-II is not critically involved in the control of reproduction. Further, the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore, we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions.
Keywordturbot GnRH brain gonad reproductive development
DOI10.1007/s00343-018-7068-y
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000441231100030
PublisherSCIENCE PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159856
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu Qinghua; Li Jun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhao Chunyan,Xu Shihong,Feng Chengcheng,et al. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1360-1373.
APA Zhao Chunyan.,Xu Shihong.,Feng Chengcheng.,Liu Yifan.,Yang Yang.,...&Li Jun.(2018).Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1360-1373.
MLA Zhao Chunyan,et al."Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1360-1373.
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