Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus | |
Zhao Chunyan1,2,3 ; Xu Shihong1,2; Feng Chengcheng1,2,3; Liu Yifan1,3; Yang Yang1,2,3; Wang Yanfeng1,3; Xiao Yongshuang1,3; Song Zongcheng4; Liu Qinghua1,3; Li Jun1,3
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| 2018-07-01 | |
| Source Publication | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 2096-5508 |
| Volume | 36Issue:4Pages:1360-1373 |
| Corresponding Author | Liu Qinghua(qinghualiu@qdio.ac.cn) ; Li Jun(junli@qdio.ac.cn) |
| Abstract | Turbots (Schophthalmus maximus), one of the most important economic marine flatfish species, fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates, reproduction is regulated by the brain-pituitary-gonadal axis (BPG-axis), and gonadotropin releasing hormone (GnRH) is one of its key components. Therefore, to better understand the physiology of reproduction in the turbot, three of the genes encoding GnRH subtypes-sbGnRH, cGnRH - II and sGnRH - were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain; sbGnRH and sGnRH mRNAs were also detected in the gonads and pituitary gland, and sbGnRH expression was much higher than that of sGnRH, indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover, the brain expression patterns of sbGnRH and sGnRH mRNAs showed an increased trend during gonadal development, peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition, cGnRH - II mRNA expression showed no significant variations, suggesting that cGnRH-II is not critically involved in the control of reproduction. Further, the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore, we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions. |
| Keyword | turbot GnRH brain gonad reproductive development |
| DOI | 10.1007/s00343-018-7068-y |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] |
| WOS Research Area | Marine & Freshwater Biology ; Oceanography |
| WOS Subject | Limnology ; Oceanography |
| WOS ID | WOS:000441231100030 |
| Publisher | SCIENCE PRESS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/159856 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu Qinghua; Li Jun |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
| First Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhao Chunyan,Xu Shihong,Feng Chengcheng,et al. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1360-1373. |
| APA | Zhao Chunyan.,Xu Shihong.,Feng Chengcheng.,Liu Yifan.,Yang Yang.,...&Li Jun.(2018).Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1360-1373. |
| MLA | Zhao Chunyan,et al."Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1360-1373. |
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| Characterization and（1896KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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