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Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)
Fan Xiaolei; Fang Yongjun; Hu Songnian; Wang Guangce; Wang, GC, Chinese Acad Sci, Inst Oceanol, IOCAS, Qingdao 266071, Peoples R China
2007-12-01
Source PublicationJOURNAL OF PHYCOLOGY
ISSN0022-3646
Volume43Issue:6Pages:1287-1294
SubtypeArticle
AbstractPorphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) is cultivated in China and widely consumed in Asia. To gain more insight into its physiological and biochemical properties, we generated 5318 expressed sequence tags (ESTs) from the sporophyte of P. haitanensis, and upon assembling into a nonredundant set, 2535 sequences were obtained, among which only 32.2% (816) shared certain similarity with published sequences (Nr and KOG). Functional classification of such ESTs revealed that most of the transcripts were related to its conservative biological metabolism, and P. haitanensis most likely possesses cyanide-resistant respiration and a C4-like carbon-fixation pathway, both of which have never been reported in a rhodophyte before. Twenty-eight percent of the nonredundant gene clusters exhibited significant similarity to those from P. yezoensis Ueda sporophytes, and 16 genes up-regulated in P. yezoensis sporophytes were also expressed abundantly in P. haitanensis. Codon usage analysis indicated that exposure to high GC pressure might occur during evolution of P. haitanensis. These findings represent the most extensive collection of ESTs from P. haitanensis to date, and all the ESTs in this study have been submitted to GenBank (accession nos. DN604790-DN608469, EG016226-EG018540).; Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) is cultivated in China and widely consumed in Asia. To gain more insight into its physiological and biochemical properties, we generated 5318 expressed sequence tags (ESTs) from the sporophyte of P. haitanensis, and upon assembling into a nonredundant set, 2535 sequences were obtained, among which only 32.2% (816) shared certain similarity with published sequences (Nr and KOG). Functional classification of such ESTs revealed that most of the transcripts were related to its conservative biological metabolism, and P. haitanensis most likely possesses cyanide-resistant respiration and a C4-like carbon-fixation pathway, both of which have never been reported in a rhodophyte before. Twenty-eight percent of the nonredundant gene clusters exhibited significant similarity to those from P. yezoensis Ueda sporophytes, and 16 genes up-regulated in P. yezoensis sporophytes were also expressed abundantly in P. haitanensis. Codon usage analysis indicated that exposure to high GC pressure might occur during evolution of P. haitanensis. These findings represent the most extensive collection of ESTs from P. haitanensis to date, and all the ESTs in this study have been submitted to GenBank (accession nos. DN604790-DN608469, EG016226-EG018540).
KeywordBioinformatic Analyses Ests Porphyra Haitanensis Rhodophyta Sporophyte
Subject AreaPlant Sciences ; Marine & Freshwater Biology
DOI10.1111/j.1529-8817.2007.00415.x
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000251416100017
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/1597
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, GC, Chinese Acad Sci, Inst Oceanol, IOCAS, Qingdao 266071, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Oceanol, IOCAS, Qingdao 266071, Peoples R China
2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China
3.Zhejiang Univ, Hangzhou Genom Inst, James D Watson Inst Genome Sci, Hangzhou 310007, Peoples R China
4.Chinese Acad Sci, Beijing Genom Inst, Beijing 101300, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
6.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Fan Xiaolei,Fang Yongjun,Hu Songnian,et al. Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)[J]. JOURNAL OF PHYCOLOGY,2007,43(6):1287-1294.
APA Fan Xiaolei,Fang Yongjun,Hu Songnian,Wang Guangce,&Wang, GC, Chinese Acad Sci, Inst Oceanol, IOCAS, Qingdao 266071, Peoples R China.(2007).Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta).JOURNAL OF PHYCOLOGY,43(6),1287-1294.
MLA Fan Xiaolei,et al."Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)".JOURNAL OF PHYCOLOGY 43.6(2007):1287-1294.
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