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Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)
Fan Xiaolei; Fang Yongjun; Hu Songnian; Wang Guangce; Wang, GC, Chinese Acad Sci, Inst Oceanol, IOCAS, Qingdao 266071, Peoples R China
2007-12-01
发表期刊JOURNAL OF PHYCOLOGY
ISSN0022-3646
卷号43期号:6页码:1287-1294
文章类型Article
摘要Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) is cultivated in China and widely consumed in Asia. To gain more insight into its physiological and biochemical properties, we generated 5318 expressed sequence tags (ESTs) from the sporophyte of P. haitanensis, and upon assembling into a nonredundant set, 2535 sequences were obtained, among which only 32.2% (816) shared certain similarity with published sequences (Nr and KOG). Functional classification of such ESTs revealed that most of the transcripts were related to its conservative biological metabolism, and P. haitanensis most likely possesses cyanide-resistant respiration and a C4-like carbon-fixation pathway, both of which have never been reported in a rhodophyte before. Twenty-eight percent of the nonredundant gene clusters exhibited significant similarity to those from P. yezoensis Ueda sporophytes, and 16 genes up-regulated in P. yezoensis sporophytes were also expressed abundantly in P. haitanensis. Codon usage analysis indicated that exposure to high GC pressure might occur during evolution of P. haitanensis. These findings represent the most extensive collection of ESTs from P. haitanensis to date, and all the ESTs in this study have been submitted to GenBank (accession nos. DN604790-DN608469, EG016226-EG018540).; Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) is cultivated in China and widely consumed in Asia. To gain more insight into its physiological and biochemical properties, we generated 5318 expressed sequence tags (ESTs) from the sporophyte of P. haitanensis, and upon assembling into a nonredundant set, 2535 sequences were obtained, among which only 32.2% (816) shared certain similarity with published sequences (Nr and KOG). Functional classification of such ESTs revealed that most of the transcripts were related to its conservative biological metabolism, and P. haitanensis most likely possesses cyanide-resistant respiration and a C4-like carbon-fixation pathway, both of which have never been reported in a rhodophyte before. Twenty-eight percent of the nonredundant gene clusters exhibited significant similarity to those from P. yezoensis Ueda sporophytes, and 16 genes up-regulated in P. yezoensis sporophytes were also expressed abundantly in P. haitanensis. Codon usage analysis indicated that exposure to high GC pressure might occur during evolution of P. haitanensis. These findings represent the most extensive collection of ESTs from P. haitanensis to date, and all the ESTs in this study have been submitted to GenBank (accession nos. DN604790-DN608469, EG016226-EG018540).
关键词Bioinformatic Analyses Ests Porphyra Haitanensis Rhodophyta Sporophyte
学科领域Plant Sciences ; Marine & Freshwater Biology
DOI10.1111/j.1529-8817.2007.00415.x
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收录类别SCI
语种英语
WOS记录号WOS:000251416100017
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被引频次:50[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/1597
专题实验海洋生物学重点实验室
通讯作者Wang, GC, Chinese Acad Sci, Inst Oceanol, IOCAS, Qingdao 266071, Peoples R China
作者单位1.Chinese Acad Sci, Inst Oceanol, IOCAS, Qingdao 266071, Peoples R China
2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China
3.Zhejiang Univ, Hangzhou Genom Inst, James D Watson Inst Genome Sci, Hangzhou 310007, Peoples R China
4.Chinese Acad Sci, Beijing Genom Inst, Beijing 101300, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
6.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
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Fan Xiaolei,Fang Yongjun,Hu Songnian,et al. Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)[J]. JOURNAL OF PHYCOLOGY,2007,43(6):1287-1294.
APA Fan Xiaolei,Fang Yongjun,Hu Songnian,Wang Guangce,&Wang, GC, Chinese Acad Sci, Inst Oceanol, IOCAS, Qingdao 266071, Peoples R China.(2007).Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta).JOURNAL OF PHYCOLOGY,43(6),1287-1294.
MLA Fan Xiaolei,et al."Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)".JOURNAL OF PHYCOLOGY 43.6(2007):1287-1294.
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