IOCAS-IR
CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1
Su, Shuonan1; Xu, Huanli1; Chen, Xiaoliang2; Qiao, Gan1; Farooqi, Ammad A.3; Tian, Ye1; Yuan, Ru1; Liu, Xiaohui1; Li, Cong1; Li, Xiao1; Wu, Ning4; Lin, Xiukun1
2018
Source PublicationRECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY
ISSN1574-8928
Volume13Issue:3Pages:360-367
Corresponding AuthorLin, Xiukun(xiukunlin@126.com)
AbstractBackground: CS5931, a novel marine peptide, was extracted and purified from the sea squirt Ciona savignyi. Our previous studies showed that recombinant CS5931 can significantly inhibit tumor growth both in vitro and in vivo. However, its molecular targets have not been elucidated. Methods: The target of the recombinant CS5931 was identified by pull-down/SDS-PAGE/MS approaches and confirmed by Western blot and surface plasmon resonance analysis. Transwell experiments were used to detect whether the recombinant CS5931 inhibited cancer migration and invasion via enolase 1. Dot blotting analysis was used to detect the effect of CS5931 on the interaction of enolase 1 and plasminogen, as well as enolase 1 and uPA/uPAR. Results: Enolase 1 was identified as the molecular target interacting with the recombinant CS5931. Transwell experiment showed that the recombinant CS5931 was able to inhibit migration and invasion of HCT116 cells and enolase 1 overexpression reversed the effects of the recombinant CS5931 on migration and invasion of cancer cells. Dot blotting analysis revealed that the recombinant CS5931 interfered with the interaction among enolase 1 and plasminogen as well as enolase 1 and uPA/uPAR. Conclusion: Our present study showed that the recombinant CS5931 could inhibit tumor invasion and matastasis via interacting with enolase 1, suggesting that the new marine polypeptide CS5931 possesses the potential to be developed as a novel anticancer agent.
KeywordEnolase1 migration and invasion protein-protein interaction recombinant CS5931
DOI10.2174/1574892813666180628170240
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of China[81773776] ; Natural Science Foundation of China[81573457] ; Natural Science Foundation of China[81774191] ; Beijing Natural Science Foundation[7172031] ; Beijing Natural Science Foundation[7172029] ; Shandong Provincial Natural Science Foundation[2016GGD03015] ; Zibo Development Program of Sci. & Tech, in Shandong, China[2016KJ100048] ; National Innovative Drug Development Project of China[2014ZX-09102043-001]
WOS Research AreaOncology ; Pharmacology & Pharmacy
WOS SubjectOncology ; Pharmacology & Pharmacy
WOS IDWOS:000441337300007
PublisherBENTHAM SCIENCE PUBL LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159687
Collection中国科学院海洋研究所
Corresponding AuthorLin, Xiukun
Affiliation1.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China
2.Shanxi Datong Univ, Sch Med, Datong 037009, Peoples R China
3.Inst Biomed & Genet Engn, Islamabad, Pakistan
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Su, Shuonan,Xu, Huanli,Chen, Xiaoliang,et al. CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1[J]. RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY,2018,13(3):360-367.
APA Su, Shuonan.,Xu, Huanli.,Chen, Xiaoliang.,Qiao, Gan.,Farooqi, Ammad A..,...&Lin, Xiukun.(2018).CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1.RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY,13(3),360-367.
MLA Su, Shuonan,et al."CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1".RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY 13.3(2018):360-367.
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