IOCAS-IR  > 实验海洋生物学重点实验室
Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda
Lv, Xinjia1,2,3; Li, Shihao1,2; Zhang, Chengsong1,2; Xiang, Jianhai1,2; Li, Fuhua1,2
2018-05-01
发表期刊MARINE DRUGS
ISSN1660-3397
卷号16期号:5页码:13
通讯作者Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
摘要As a kind of antimicrobial peptides (AMP) in crustacean, anti-lipopolysaccharide factors (ALFs) have broad spectrum antimicrobial activities. In the present study, we identified four ALF genes, EcALF2-5, from the ridgetail prawn Exopalaemon carinicauda. Tissue distribution analysis showed that EcALF2 and EcALF4 transcripts were mainly located in gill, epidermis, and stomach, while EcALF3 and EcALF5 were mainly in hemocytes. Peptides corresponding to the LPS binding domain (LBD) of EcALFs were synthesized for analyzing their antimicrobial activities. Minimal inhibitory concentration (MIC) analysis showed that the synthetic LBD peptides of EcALF3 and EcALF4 could inhibit the growth of Gram-positive and Gram-negative bacteria, while the synthetic LBD peptides of EcALF2 and EcALF5 showed antibacterial activity against Vibrio. Incubation of white spot syndrome virus (WSSV) with the synthetic LBD peptides of EcALF3, EcALF4, and EcALF5 could reduce the in vivo viral copy number in WSSV-infected prawns. After silencing of EcALFs, Vibrio exhibited a rapid proliferation in the hepatopancreas of the prawn. The present data showed the important function of different EcALFs in modulating the in vivo bacterial and viral propagation in E. carinicauda. This study will provide new clues into the disease control in aquaculture.
关键词anti-lipopolysaccharide factor antimicrobial RNA interference flora balance crustacean
DOI10.3390/md16050145
收录类别SCI
语种英语
资助项目General Program of National Natural Science Foundation of China[41376165] ; General Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; China Agriculture Research system-48 (CARS-48) ; General Program of National Natural Science Foundation of China[41376165] ; General Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; China Agriculture Research system-48 (CARS-48) ; General Program of National Natural Science Foundation of China[41376165] ; General Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; China Agriculture Research system-48 (CARS-48)
WOS研究方向Pharmacology & Pharmacy
WOS类目Chemistry, Medicinal
WOS记录号WOS:000435195100008
出版者MDPI
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/159504
专题实验海洋生物学重点实验室
通讯作者Li, Shihao; Li, Fuhua
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Lv, Xinjia,Li, Shihao,Zhang, Chengsong,et al. Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda[J]. MARINE DRUGS,2018,16(5):13.
APA Lv, Xinjia,Li, Shihao,Zhang, Chengsong,Xiang, Jianhai,&Li, Fuhua.(2018).Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda.MARINE DRUGS,16(5),13.
MLA Lv, Xinjia,et al."Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda".MARINE DRUGS 16.5(2018):13.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Multiple Isoforms of(3155KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lv, Xinjia]的文章
[Li, Shihao]的文章
[Zhang, Chengsong]的文章
百度学术
百度学术中相似的文章
[Lv, Xinjia]的文章
[Li, Shihao]的文章
[Zhang, Chengsong]的文章
必应学术
必应学术中相似的文章
[Lv, Xinjia]的文章
[Li, Shihao]的文章
[Zhang, Chengsong]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。