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Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino
Gao, Xiaolong1; Zhang, Mo1; Li, Xian1; Han, Yin2; Wu, Fucun1; Liu, Ying3
2018-05-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume76Pages:143-152
Corresponding AuthorLi, Xian(lixian@qdio.ac.cn) ; Liu, Ying(yingliu@dlou.edu.cn)
AbstractTo study the effects of a probiotic (Bacillus lincheniformis) on the survival and growth of Minds discus hannai Ino, the expression levels of nonspecific immune genes and the resistance to Vibrio parahaemolyticus infection were assessed. Abalones (shell length: 27.64 +/- 1.59 mm, body weight: 4.17 +/- 0.32 g) were selected for use in an 8 week culture experiment and a 2-week V. parahaemolyticus artificial infection experiment. In both experiments, the control group (C) was fed with a basal feed and the experimental groups were fed with experimental food prepared by spraying the probiotic on the basal feed at different concentrations: 10(3) (B1), 10(5) (B2), and 10(7) (B3) cfu/mL. The survival rate, total number of blood lymphocytes, activity of acid phosphatase, and expression level of heat shock protein 70 were significantly higher in B1, B2, and B3 than in C (P < 0.05). The specific growth rate of shell length, food intake, food conversion rate, phagocytic activity of blood lymphocytes, activities of myeloperoxidase and catalase (CAT), and expression levels of CAT and thioredoxin peroxidase of abalones in B2 were significantly higher than those in B1 and C (P < 0.05). Although the level of O-2(-) produced by the respiratory burst of blood lymphocytes in B2 was not significantly different from those in B1 and B3, they were significantly higher than that in C (P < 0.05). The activity of superoxide dismutase (SOD), the nitric oxide levels produced by the respiratory burst of blood lymphocytes, and the expression levels of Mn-SOD in B1 and B3 were significantly higher than those in C but significantly lower than those in B2 (P < 0.05). Fourteen days after infection with V. parahaemolyticus, the cumulative mortality of abalones in B2 was significantly lower than those in B1 and C (P < 0.05). These results indicate that the food containing 10(5) cfu/mL Bacillus licheniformis promoted food intake and growth of abalones and also improved their resistance to V. parahaemolyticus infection. Thus, B. licheniformis is a good potential probiotic.
KeywordBacillus licheniformis Haliotis discus hannai Ino Growth Immunity Disease resistance
DOI10.1016/j.fsi.2018.02.028
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFB0404000] ; earmarked fund for the Modern Agro-industry Technology Research System[CARS-48] ; National Natural Science Fund of China[31672673] ; National Natural Science Fund of China[31402283] ; National Natural Science Fund of China[41306152]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000430888400017
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159416
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Xian; Liu, Ying
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Ocean Univ China, Qingdao 266003, Peoples R China
3.Dalian Ocean Univ, Dalian 116023, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Gao, Xiaolong,Zhang, Mo,Li, Xian,et al. Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino[J]. FISH & SHELLFISH IMMUNOLOGY,2018,76:143-152.
APA Gao, Xiaolong,Zhang, Mo,Li, Xian,Han, Yin,Wu, Fucun,&Liu, Ying.(2018).Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino.FISH & SHELLFISH IMMUNOLOGY,76,143-152.
MLA Gao, Xiaolong,et al."Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino".FISH & SHELLFISH IMMUNOLOGY 76(2018):143-152.
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