Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino | |
Gao, Xiaolong1 ; Zhang, Mo1; Li, Xian1; Han, Yin2; Wu, Fucun1; Liu, Ying3
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| 2018-05-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| Volume | 76Pages:143-152 |
| Corresponding Author | Li, Xian(lixian@qdio.ac.cn) ; Liu, Ying(yingliu@dlou.edu.cn) |
| Abstract | To study the effects of a probiotic (Bacillus lincheniformis) on the survival and growth of Minds discus hannai Ino, the expression levels of nonspecific immune genes and the resistance to Vibrio parahaemolyticus infection were assessed. Abalones (shell length: 27.64 +/- 1.59 mm, body weight: 4.17 +/- 0.32 g) were selected for use in an 8 week culture experiment and a 2-week V. parahaemolyticus artificial infection experiment. In both experiments, the control group (C) was fed with a basal feed and the experimental groups were fed with experimental food prepared by spraying the probiotic on the basal feed at different concentrations: 10(3) (B1), 10(5) (B2), and 10(7) (B3) cfu/mL. The survival rate, total number of blood lymphocytes, activity of acid phosphatase, and expression level of heat shock protein 70 were significantly higher in B1, B2, and B3 than in C (P < 0.05). The specific growth rate of shell length, food intake, food conversion rate, phagocytic activity of blood lymphocytes, activities of myeloperoxidase and catalase (CAT), and expression levels of CAT and thioredoxin peroxidase of abalones in B2 were significantly higher than those in B1 and C (P < 0.05). Although the level of O-2(-) produced by the respiratory burst of blood lymphocytes in B2 was not significantly different from those in B1 and B3, they were significantly higher than that in C (P < 0.05). The activity of superoxide dismutase (SOD), the nitric oxide levels produced by the respiratory burst of blood lymphocytes, and the expression levels of Mn-SOD in B1 and B3 were significantly higher than those in C but significantly lower than those in B2 (P < 0.05). Fourteen days after infection with V. parahaemolyticus, the cumulative mortality of abalones in B2 was significantly lower than those in B1 and C (P < 0.05). These results indicate that the food containing 10(5) cfu/mL Bacillus licheniformis promoted food intake and growth of abalones and also improved their resistance to V. parahaemolyticus infection. Thus, B. licheniformis is a good potential probiotic. |
| Keyword | Bacillus licheniformis Haliotis discus hannai Ino Growth Immunity Disease resistance |
| DOI | 10.1016/j.fsi.2018.02.028 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2017YFB0404000] ; earmarked fund for the Modern Agro-industry Technology Research System[CARS-48] ; National Natural Science Fund of China[31672673] ; National Natural Science Fund of China[31402283] ; National Natural Science Fund of China[41306152] ; National Key R&D Program of China[2017YFB0404000] ; earmarked fund for the Modern Agro-industry Technology Research System[CARS-48] ; National Natural Science Fund of China[31672673] ; National Natural Science Fund of China[31402283] ; National Natural Science Fund of China[41306152] |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000430888400017 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/159416 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Li, Xian; Liu, Ying |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Ocean Univ China, Qingdao 266003, Peoples R China 3.Dalian Ocean Univ, Dalian 116023, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Gao, Xiaolong,Zhang, Mo,Li, Xian,et al. Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino[J]. FISH & SHELLFISH IMMUNOLOGY,2018,76:143-152. |
| APA | Gao, Xiaolong,Zhang, Mo,Li, Xian,Han, Yin,Wu, Fucun,&Liu, Ying.(2018).Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino.FISH & SHELLFISH IMMUNOLOGY,76,143-152. |
| MLA | Gao, Xiaolong,et al."Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino".FISH & SHELLFISH IMMUNOLOGY 76(2018):143-152. |
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| Effects of a probiot（683KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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