An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development

doi:10.1016/j.gene.2018.04.035

IOCAS-IR > 实验海洋生物学重点实验室

	An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development
	Tan, Sujian 1,3; Huan, Pin 1; Liu, Baozhong1,2
	2018-07-15
发表期刊	GENE
ISSN	0378-1119
卷号	663 页码:65-71
通讯作者	Huan, Pin(huanpin@qdio.ac.cn)
摘要	Though the roles of BMP signaling in development is studied extensively in insects and vertebrates, our knowledge of BMP signaling in molluscan development is limited. In the present study, we performed a genome-based analysis of TGF-beta receptors in the Pacific oyster Crassostrea gigas and revealed that C. gigas possessed all five canonical members of the gene family, including three type I and two type II receptors. Whole mount in situ hybridization revealed that four receptor genes exhibited universal expression at the gastrula stage but cgi-bmprll mRNA was only expressed at the anterior lip of blastopore, indicating the regulation of BMP signaling through restricted expression of TGF-beta receptors. Treatment of oyster embryos using a BMP signaling inhibitor (dorsomorphin) resulted in obvious changes of the expression of developmental regulatory genes. In particular, three dorsally expressed genes were inhibited and two genes expressed at the opposite side showed increased expression, indicating BMP signaling may function in dorsal-ventral (DV) patterning. Some of the influenced genes were potential shell-formation (pSF) genes, including the well-accepted pSF gene engrailed, and thus this indicates the development of shell field was affected by dorsomorphin treatment. Subsequent scanning electronic observation revealed morphological change of the posterior margin of the shell field. Taken together, these results indicate the roles of BMP signaling in both DV patterning and development of shell field in oyster embryo, which expands our knowledge of early molluscan development.
关键词	BMP signaling Dorsomorphin Dorsal-ventral patterning Shell field Mollusc
DOI	10.1016/j.gene.2018.04.035
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China (NSFC)[31472265] ; National Natural Science Foundation of China (NSFC)[41776157] ; National Natural Science Foundation of China (NSFC)[31472265] ; National Natural Science Foundation of China (NSFC)[41776157]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000434901700008
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/159372
专题	实验海洋生物学重点实验室
通讯作者	Huan, Pin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Tan, Sujian,Huan, Pin,Liu, Baozhong. An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development[J]. GENE,2018,663:65-71.
APA	Tan, Sujian,Huan, Pin,&Liu, Baozhong.(2018).An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development.GENE,663,65-71.
MLA	Tan, Sujian,et al."An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development".GENE 663(2018):65-71.

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