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Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas
She, Zhicai3; Li, Li1,2,5; Meng, Jie1,2,5; Jia, Zhen3; Que, Huayong1,5; Zhang, Guofan1,4,5
2018-06-06
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume8Pages:10
Corresponding AuthorLi, Li(lili@qdio.ac.cn) ; Zhang, Guofan(gfzhang@qdio.ac.cn)
AbstractThe Pacific oyster Crassostrea gigas is an important cultivated shellfish. As a euryhaline species, it has evolved adaptive mechanisms responding to the complex and changeable intertidal environment that it inhabits. To investigate the genetic basis of this salinity adaptation mechanism, we conducted a genome-wide association study using phenotypically differentiated populations (hyposalinity and hypersalinity adaptation populations, and control population), and confirmed our results using an independent population, high-resolution melting, and mRNA expression analysis. For the hyposalinity adaptation, we determined 24 genes, including Cg_CLCN7 (chloride channel protein 7) and Cg_AP1 (apoptosis 1 inhibitor), involved in the ion/water channel and transporter mechanisms, free amino acid and reactive oxygen species metabolism, immune responses, and chemical defence. Three SNPs located on these two genes were significantly differentiated between groups, as was Cg_CLCN7. For the hypersalinity adaptation, the biological process for positive regulating the developmental process was enriched. Enriched gene functions were focused on transcriptional regulation, signal transduction, and cell growth and differentiation, including calmodulin (Cg_CaM) and ficolin-2 (Cg_FCN2). These genes and polymorphisms possibly play an important role in oyster hyposalinity and hypersalinity adaptation. They not only further our understanding of salinity adaptation mechanisms but also provide markers for highly adaptable oyster strains suitable for breeding.
DOI10.1038/s41598-018-26953-w
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31572620] ; National Natural Science Foundation of China[31530079] ; National Basic Research Program of China (973 Program)[2010CB126402] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] ; Education Department Program of Guangxi[KY2016YB490] ; Key Laboratory of Beibu Gulf Marine Biodiversity Conservation Program of Guangxi[2018ZB07]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000434252000019
PublisherNATURE PUBLISHING GROUP
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159237
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Li; Zhang, Guofan
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China
3.Qinzhou Univ, Guangxi Key Lab Beibu Gulf Marine Biodivers Conse, Qinzhou 535011, Guangxi, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
She, Zhicai,Li, Li,Meng, Jie,et al. Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas[J]. SCIENTIFIC REPORTS,2018,8:10.
APA She, Zhicai,Li, Li,Meng, Jie,Jia, Zhen,Que, Huayong,&Zhang, Guofan.(2018).Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas.SCIENTIFIC REPORTS,8,10.
MLA She, Zhicai,et al."Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas".SCIENTIFIC REPORTS 8(2018):10.
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