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Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas
Jiang, Shuai1; Qiu, Limei1; Wang, Lingling2; Jia, Zhihao1,3; Lv, Zhao1,3; Wang, Mengqiang1; Liu, Conghui1,3; Xu, Jiachao1,3; Song, Linsheng2
2018-06-11
Source PublicationFRONTIERS IN IMMUNOLOGY
ISSN1664-3224
Volume9Pages:17
Corresponding AuthorWang, Lingling(wanglingling@dlou.edu.cn)
AbstractAs invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of this type from the Pacific oyster Crassostrea gigas were classified efficiently in this study via fluorescence-activated cell sorting (FACS) based on their phagocytosis of FITC-labeled latex beads. Transcriptomic and quantitative proteomic analyses revealed a series of differentially expressed genes (DEGs) and proteins present in phagocytes; of the 352 significantly high expressed proteins identified here within the phagocyte proteome, 262 corresponding genes were similarly high expressed in the transcriptome, while 140 of 205 significantly low expressed proteins within the proteome were transcriptionally low expressed. A pathway crosstalk network analysis of these significantly high expressed proteins revealed that phagocytes were highly activated in a number of antimicrobial-related biological processes, including oxidation-reduction and lysosomal proteolysis processes. A number of DEGs, including oxidase, lysosomal protease, and immune receptors, were also validated in this study using quantitative PCR, while seven lysosomal cysteine proteases, referred to as cathepsin Ls, were significantly high expressed in phagocytes. Results show that the expression level of cathepsin L protein in phagocytes [mean fluorescence intensity (MFI): 327 +/- 51] was significantly higher (p < 0.01) than that in non-phagocytic hemocytes (MFI: 83 +/- 26), while the cathepsin L protein was colocalized with the phagocytosed Vibrio splendidus in oyster hemocytes during this process. The results of this study collectively suggest that oyster phagocytes possess both potent oxidative killing and microbial disintegration capacities; these findings provide important insights into hemocyte phagocytic killing as a component of C. gigas innate immunity.
KeywordCrassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L
DOI10.3389/fimmu.2018.01280
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of China[31530069] ; Natural Science Foundation of China[41406170] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; 863 Program[2014AA103501] ; for Modern Agro-industry Technology Research System[CARS-49] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000434796400001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159203
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lingling
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jiang, Shuai,Qiu, Limei,Wang, Lingling,et al. Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2018,9:17.
APA Jiang, Shuai.,Qiu, Limei.,Wang, Lingling.,Jia, Zhihao.,Lv, Zhao.,...&Song, Linsheng.(2018).Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.FRONTIERS IN IMMUNOLOGY,9,17.
MLA Jiang, Shuai,et al."Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas".FRONTIERS IN IMMUNOLOGY 9(2018):17.
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