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Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection
Li, Mo-fei1,2; Sun, Li1,2
2018-05-25
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume9Pages:10
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractEdwardsiella tarda is a broad-host pathogen that can infect mammals, reptiles, and fish. E. tarda exhibits a remarkable ability to survive in host serum and replicate in host phagocytes, but the underlining mechanism is unclear. In this study, in order to identify E. tarda proteins involved in serum resistance, iTRAQ proteomic analysis was performed to examine the whole-cell protein profiles of TX01, a pathogenic E. tarda isolate, in response to serum treatment. Of the differentially expressed proteins identified, one (named Sip2) possesses a conserved hydrogenase domain and is homologous to the putative hydrogenase accessory protein HypB. When Sip2 was expressed in Escherichia coli, it significantly enhanced the survival of the host cells in serum. Compared to TX01, the sip2 knockout, TX01 Delta sip2, was dramatically reduced in the ability of hydrogenase activity, serum resistance, intracellular replication, dissemination in fish tissues, and causing mortality in infected fish. The lost virulence capacities of TX01 Delta sip2 were restored by complementation with the sip2 gene. Furthermore, TX01 Delta sip2 was significantly reduced in the capacity to grow under low pHs and iron-depleted conditions, and was unable to maintain its internal pH in acidic environment. Taken together, these results indicate that Sip2 is a novel serum-induced protein that is essential to serum resistance, cellular and tissue infection, and coping with acidic stress via its ability to modulate intracellular pH.
KeywordEdwardsiella tarda hydrogenase serum resistance acid resistance virulence
DOI10.3389/fmicb.2018.01084
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31602197] ; National Natural Science Foundation of China[31330081] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2017249] ; Huiquan Young Scholar Program of Institute of Oceanology, CAS ; Taishan Scholar Program of Shandong Province
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000433133300001
PublisherFRONTIERS MEDIA SA
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159158
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Mo-fei,Sun, Li. Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection[J]. FRONTIERS IN MICROBIOLOGY,2018,9:10.
APA Li, Mo-fei,&Sun, Li.(2018).Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection.FRONTIERS IN MICROBIOLOGY,9,10.
MLA Li, Mo-fei,et al."Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection".FRONTIERS IN MICROBIOLOGY 9(2018):10.
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