IOCAS-IR  > 实验海洋生物学重点实验室
Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity
Geng, Lihua1,2,3; Zhang, Quanbin1,3; Wang, Jing1,3; Jin, Weihua4; Zhao, Tingting5; Hu, Weicheng6
2018-07-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume113Pages:90-97
Corresponding AuthorZhang, Quanbin(qbzhang@qdio.ac.cn)
AbstractCrude polysaccharide obtained from Saccharina japonica using acid hydrolysis and precipitation was separated into sulfated fuco-oligosaccharide (HDF1) and heteropolysaccharide (HDF2). To further explore the bioactive fraction, HDF2 was successfully separated using membrane filtration into HDF2A and HDF2B, which differed in chemical composition and molecular weight. The bioactivity of all the fractions was tested in vitro, including immunomodulatory activity in RAW 264.7 cells and the protective activity in aristolochic acid (AA)-induced NRK-52E cell injury. HDF1 and HDF2B (low-molecular weight sulfated fucans/fuco-oligosaccharides) did not increase the nitric oxide production in RAW 264.7 cells, whereas HDF2 and HDF2A exhibited potential immunomodulatory activity. All the tested compounds showed different degrees of protective activity in AA-induced injury; HDF2A exhibited superior protective activity. Through chemical analysis, HPLC analysis, and IR spectroscopy and MS, it was determined that HDF2A was a galactose-enriched heteropolysaccharide- glucofucogalactan with a distinctive 2:1 ratio of galactose to fucose, In addition, HDF2A also contained a high amount of glucose and minor amounts of mannose, rhamnose, and xylose, with a low content of sulfate. Thus, HDF2A, a complex heterogeneous polysaccharide mixture with a unique monosaccharide composition, could be studied for further structural characterization and pharmaceutical applications. (C) 2018 Elsevier B.V. All rights reserved.
KeywordSaccharina japonica Heteropolysaccharide Aristolochic acid Immunomodulation
DOI10.1016/j.ijbiomac.2018.02.008
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of China[41376166] ; Natural Science Foundation of China[41406144] ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Key Research and Development Project of Shandong Province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190] ; Science and Technology project of Fujian Province[201773015] ; K.C. Wong Education Foundation
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000432503100012
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159151
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Quanbin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310014, Zhejiang, Peoples R China
5.China Japan Friendship Hosp, Inst Clin Med Sci, Beijing Key Lab Immune Mediated Inflammatory Dis, Beijing, Peoples R China
6.Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Key Lab Ecoagr Biotechnol Around Hongze L, Huaian 223300, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Geng, Lihua,Zhang, Quanbin,Wang, Jing,et al. Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,113:90-97.
APA Geng, Lihua,Zhang, Quanbin,Wang, Jing,Jin, Weihua,Zhao, Tingting,&Hu, Weicheng.(2018).Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,113,90-97.
MLA Geng, Lihua,et al."Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 113(2018):90-97.
Files in This Item:
File Name/Size DocType Version Access License
Glucofucogalactan, a(1252KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Geng, Lihua]'s Articles
[Zhang, Quanbin]'s Articles
[Wang, Jing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Geng, Lihua]'s Articles
[Zhang, Quanbin]'s Articles
[Wang, Jing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Geng, Lihua]'s Articles
[Zhang, Quanbin]'s Articles
[Wang, Jing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.