Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China

doi:10.1111/j.1365-2672.2007.03494.x

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	Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China
	Dang, H.; Zhang, X.; Song, L.; Chang, Y.; Yang, G.; Dang, H, Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2007-12-01
发表期刊	JOURNAL OF APPLIED MICROBIOLOGY
ISSN	1364-5072
卷号	103 期号:6 页码:2580-2592
文章类型	Article
摘要	Aims: To assess the diversity of antibiotic-resistant bacteria and their resistance genes in typical maricultural environments.; Aims: To assess the diversity of antibiotic-resistant bacteria and their resistance genes in typical maricultural environments. Methods nand Results: Multidrug-resistant bacteria and resistance genes from a mariculture farm of China were analysed via cultivation and polymerase chain reaction (PCR) methods. Oxytetracycline (OTC)-resistant bacteria were abundant in both abalone and turbot rearing waters, accounting for 3.7% and 9.9% of the culturable microbes. Multidrug resistance was common, with simultaneous resistance to OTC, chloramphenicol and ampicillin the most common resistance phenotype. 16S rDNA sequence analyses indicate that the typical resistant isolates belonged to marine Vibrio, Pseudoalteromonas or Alteromonas species, with resistance most common in Vibrio splendidus isolates. For OTC resistance, tet(A), tet(B) and tet(M) genes were detected in some multidrug-resistant isolates, with tet(D) being the most common molecular determinant. For chloramphenicol resistance, cat II was common, and floR was also detected, especially in marine Pseudoalteromonas strains. Conclusions: There is the risk of multidrug-resistant bacteria contamination in mariculture environments and marine Vibrio and Pseudoalteromonas species serve as reservoirs of specific antibiotic resistance determinants. Significance and Impact of the Study: This paper and similar findings from Korea and Japan indicate the potential for widespread distribution of antibiotic resistance genes in mariculture environments from the East Asian region of the world.
关键词	Antibiotic Resistance Cat Gene Flor Gene Mariculture Environment Marine Pseudoalteromonas Marine Vibrios Tet Gene Vibrio Splendidus
学科领域	Biotechnology & Applied Microbiology ; Microbiology
DOI	10.1111/j.1365-2672.2007.03494.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000251251200061
引用统计	被引频次：68[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1589
专题	实验海洋生物学重点实验室
通讯作者	Dang, H, Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 2.Ocean Univ China, Life Sci & Technol Coll, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao, Peoples R China 4.Dalian Fisheries Univ, Key Lab Mariculture & Biotechnol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Dang, H.,Zhang, X.,Song, L.,et al. Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China[J]. JOURNAL OF APPLIED MICROBIOLOGY,2007,103(6):2580-2592.
APA	Dang, H.,Zhang, X.,Song, L.,Chang, Y.,Yang, G.,&Dang, H, Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2007).Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China.JOURNAL OF APPLIED MICROBIOLOGY,103(6),2580-2592.
MLA	Dang, H.,et al."Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China".JOURNAL OF APPLIED MICROBIOLOGY 103.6(2007):2580-2592.

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