Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies | |
Han, Yu1,2,3; Zang, Kun1,2,3; Liu, Changshui1,2; Li, Yingjie1,2; Ma, Qingjun1,2 | |
2018-05-01 | |
发表期刊 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN | 2053-230X |
卷号 | 74页码:283-287 |
通讯作者 | Ma, Qingjun(qma@qdio.ac.cn) |
摘要 | Siderophore-interacting proteins (SIPs) play an important role in iron acquisition in many bacteria. SIPs release iron from the internalized ferric siderophore complex by reducing ferric iron to ferrous iron, but how the iron is reduced is not well understood. Here, a sip gene was identified in the genome of Vibrio anguillarum 775. To further understand the catalytic mechanism of the protein, the SIP was overexpressed in Escherichia coli Rosetta (DE3) cells, purified and crystallized for X-ray diffraction analysis. The crystal diffracted to 1.113 angstrom resolution and belonged to space group P2(1), with unit-cell parameters a = 64.63, b = 58.47, c = 70.65 angstrom, beta= 114.19 degrees. |
关键词 | Vibrio anguillarum siderophore-interacting proteins iron release siderophores |
DOI | 10.1107/S2053230X18005125 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | 1000 Talents Program of China ; 100 Talents Program of the Chinese Academy of Sciences ; AoShan Talents Program of Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; 1000 Talents Program of China ; 100 Talents Program of the Chinese Academy of Sciences ; AoShan Talents Program of Qingdao National Laboratory for Marine Science and Technology[2015ASTP] |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
WOS记录号 | WOS:000431249400003 |
出版者 | INT UNION CRYSTALLOGRAPHY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/158987 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Ma, Qingjun |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Han, Yu,Zang, Kun,Liu, Changshui,et al. The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2018,74:283-287. |
APA | Han, Yu,Zang, Kun,Liu, Changshui,Li, Yingjie,&Ma, Qingjun.(2018).The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,74,283-287. |
MLA | Han, Yu,et al."The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 74(2018):283-287. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
The putative siderop(193KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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