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The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies
Han, Yu1,2,3; Zang, Kun1,2,3; Liu, Changshui1,2; Li, Yingjie1,2; Ma, Qingjun1,2
2018-05-01
发表期刊ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
ISSN2053-230X
卷号74页码:283-287
通讯作者Ma, Qingjun(qma@qdio.ac.cn)
摘要Siderophore-interacting proteins (SIPs) play an important role in iron acquisition in many bacteria. SIPs release iron from the internalized ferric siderophore complex by reducing ferric iron to ferrous iron, but how the iron is reduced is not well understood. Here, a sip gene was identified in the genome of Vibrio anguillarum 775. To further understand the catalytic mechanism of the protein, the SIP was overexpressed in Escherichia coli Rosetta (DE3) cells, purified and crystallized for X-ray diffraction analysis. The crystal diffracted to 1.113 angstrom resolution and belonged to space group P2(1), with unit-cell parameters a = 64.63, b = 58.47, c = 70.65 angstrom, beta= 114.19 degrees.
关键词Vibrio anguillarum siderophore-interacting proteins iron release siderophores
DOI10.1107/S2053230X18005125
收录类别SCI
语种英语
资助项目1000 Talents Program of China ; 100 Talents Program of the Chinese Academy of Sciences ; AoShan Talents Program of Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; 1000 Talents Program of China ; 100 Talents Program of the Chinese Academy of Sciences ; AoShan Talents Program of Qingdao National Laboratory for Marine Science and Technology[2015ASTP]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS记录号WOS:000431249400003
出版者INT UNION CRYSTALLOGRAPHY
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/158987
专题实验海洋生物学重点实验室
通讯作者Ma, Qingjun
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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Han, Yu,Zang, Kun,Liu, Changshui,et al. The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2018,74:283-287.
APA Han, Yu,Zang, Kun,Liu, Changshui,Li, Yingjie,&Ma, Qingjun.(2018).The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,74,283-287.
MLA Han, Yu,et al."The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 74(2018):283-287.
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