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The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies
Han, Yu1,2,3; Zang, Kun1,2,3; Liu, Changshui1,2; Li, Yingjie1,2; Ma, Qingjun1,2
2018-05-01
Source PublicationACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
ISSN2053-230X
Volume74Pages:283-287
Corresponding AuthorMa, Qingjun(qma@qdio.ac.cn)
AbstractSiderophore-interacting proteins (SIPs) play an important role in iron acquisition in many bacteria. SIPs release iron from the internalized ferric siderophore complex by reducing ferric iron to ferrous iron, but how the iron is reduced is not well understood. Here, a sip gene was identified in the genome of Vibrio anguillarum 775. To further understand the catalytic mechanism of the protein, the SIP was overexpressed in Escherichia coli Rosetta (DE3) cells, purified and crystallized for X-ray diffraction analysis. The crystal diffracted to 1.113 angstrom resolution and belonged to space group P2(1), with unit-cell parameters a = 64.63, b = 58.47, c = 70.65 angstrom, beta= 114.19 degrees.
KeywordVibrio anguillarum siderophore-interacting proteins iron release siderophores
DOI10.1107/S2053230X18005125
Indexed BySCI
Language英语
Funding Project1000 Talents Program of China ; 100 Talents Program of the Chinese Academy of Sciences ; AoShan Talents Program of Qingdao National Laboratory for Marine Science and Technology[2015ASTP]
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS IDWOS:000431249400003
PublisherINT UNION CRYSTALLOGRAPHY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158987
Collection实验海洋生物学重点实验室
Corresponding AuthorMa, Qingjun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Han, Yu,Zang, Kun,Liu, Changshui,et al. The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2018,74:283-287.
APA Han, Yu,Zang, Kun,Liu, Changshui,Li, Yingjie,&Ma, Qingjun.(2018).The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,74,283-287.
MLA Han, Yu,et al."The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 74(2018):283-287.
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