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Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205)
Zhao, Chunyan1,2,3; Liu, Qinghua1,3; Xu, Shihong1,3; Xiao, Yongshuang1,3; Wang, Wenqi4; Yang, Jingkun4; Yang, Yang1,2,3; Wang, Yanfeng1,3; Song, Zongcheng5; Li, Jun1,3
2018-06-01
Source PublicationTHERIOGENOLOGY
ISSN0093-691X
Volume113Pages:137-145
Corresponding AuthorLiu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
AbstractTurbot (Schophthalmus maximus) is one of the most important economic marine flatfish species. However, due to rapid development of the industry, genetic resource recession has brought down the efficiency of aquaculture. Therefore, conservation of the genetic resource is increasingly demanded. Recent research proved that type A spermatogonia possesses the properties of spermatogonia stem cell, and it might provide an ideal solution. Therefore, it is necessary to develop an appropriate molecular marker on type A spermatogonia to further isolate and purify of type A spermatogonia in turbot. In this study, turbot lymphocyte antigen 75 (smly75) gene was identified and its localizations of expressions and the temporal transcription patterns were evaluated qualitatively and semiquantitatively. Investigation in testes of development of spermatogonia showed that smly75 mRNA, contrast with vasa and dnd mRNA, was exclusively localized in type A spermatogonia and not detected in type B spermatogonia, spermatocytes or gonadal somatic cells by in situ hybridization. Thus, the smly75 could be a new and convincing molecular marker on identification of type A spermatogonia. In addition, specifically to development pattern of type A spermatogonia, from 7- to 14- month testes, spermatogonia were dominated and the number of type A spermatogonia was increased, corresponding that smly75 expression was up-regulated gradually, while, in 16 month testes, accompanied by that several spermatogonia differentiated into primary spermatocytes, the smly75 expression down-regulated. Finally, broaden in the whole reproductive cycle, the smly75 transcription significantly variated with the differentiation of germ cells and in accordance with the number of type A spermatogonia. It is suggested that testes from 8 to 14 month old males could be used for further isolation and purification of type A-SG. These results will not only help to better understand type A spermatogonia, but also further facilitate type A spematogonia-mediated germ cell manipulation in turbot. (C) 2018 Elsevier Inc. All rights reserved.
KeywordTurbot Lymphocyte antigen 75 (ly75) Testis Spermatogonia Gonadal development
DOI10.1016/j.theriogenology.2017.12.016
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31372514] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; Modern Agro-Industry Technology Research System[nycytx-50] ; China Postdoctoral Science Foundation[2014M551973] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of science and technology service network planning[KFJ-EW-STS-060]
WOS Research AreaReproductive Biology ; Veterinary Sciences
WOS SubjectReproductive Biology ; Veterinary Sciences
WOS IDWOS:000431091900018
PublisherELSEVIER SCIENCE INC
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158967
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Qinghua; Li, Jun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
4.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Peoples R China
5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhao, Chunyan,Liu, Qinghua,Xu, Shihong,et al. Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205)[J]. THERIOGENOLOGY,2018,113:137-145.
APA Zhao, Chunyan.,Liu, Qinghua.,Xu, Shihong.,Xiao, Yongshuang.,Wang, Wenqi.,...&Li, Jun.(2018).Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205).THERIOGENOLOGY,113,137-145.
MLA Zhao, Chunyan,et al."Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205)".THERIOGENOLOGY 113(2018):137-145.
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