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Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure
Zeng, Yan1,2; Zhang, Dun1,2; Qi, Peng1,2; Zheng, Laibao1,2,3
2018-07-15
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume265Pages:468-475
Corresponding AuthorZhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn)
AbstractLabel free analysis of multiplex analytes is of great demand in modern bio-analytical science. In this study, a novel DNA junction coupled with two different G-quadruplex DNA structures was demonstrated, achieving colorimetric and fluorescent signal report. Upon the introduction of different targets (including DNA and Lysozyme), the specific interaction between targets and sensing DNA junction could induce the reaction of exonuclease-stimulated recycling of target DNA and conformation transformation based on aptamer/lysozyme binding. With the integration of G-quadruplex/Hemin as colorimetric probe and G-quadruplex/PPIX as fluorescence probe, this label-free sensing system showed high sensitivity and selectivity for DNA and lysozyme detection. Furthermore, we also demonstrated that this novel sensing method could respond to these two targets independently and simultaneously, promising a great hope for its potential application in analytical fields. (C) 2018 Elsevier B.V. All rights reserved.
KeywordLabel free DNA junction G-quadruplex DNA DNA detection Lysozyme detection
DOI10.1016/j.snb.2018.03.039
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; China Postdoctoral Science Foundation[2016 M602199] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-ES02]
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000430232500057
PublisherELSEVIER SCIENCE SA
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158939
Collection海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorZhang, Dun; Qi, Peng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zeng, Yan,Zhang, Dun,Qi, Peng,et al. Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,265:468-475.
APA Zeng, Yan,Zhang, Dun,Qi, Peng,&Zheng, Laibao.(2018).Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure.SENSORS AND ACTUATORS B-CHEMICAL,265,468-475.
MLA Zeng, Yan,et al."Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure".SENSORS AND ACTUATORS B-CHEMICAL 265(2018):468-475.
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