Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure | |
Zeng, Yan1,2 ; Zhang, Dun1,2; Qi, Peng1,2; Zheng, Laibao1,2,3
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| 2018-07-15 | |
| Source Publication | SENSORS AND ACTUATORS B-CHEMICAL
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| ISSN | 0925-4005 |
| Volume | 265Pages:468-475 |
| Corresponding Author | Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn) |
| Abstract | Label free analysis of multiplex analytes is of great demand in modern bio-analytical science. In this study, a novel DNA junction coupled with two different G-quadruplex DNA structures was demonstrated, achieving colorimetric and fluorescent signal report. Upon the introduction of different targets (including DNA and Lysozyme), the specific interaction between targets and sensing DNA junction could induce the reaction of exonuclease-stimulated recycling of target DNA and conformation transformation based on aptamer/lysozyme binding. With the integration of G-quadruplex/Hemin as colorimetric probe and G-quadruplex/PPIX as fluorescence probe, this label-free sensing system showed high sensitivity and selectivity for DNA and lysozyme detection. Furthermore, we also demonstrated that this novel sensing method could respond to these two targets independently and simultaneously, promising a great hope for its potential application in analytical fields. (C) 2018 Elsevier B.V. All rights reserved. |
| Keyword | Label free DNA junction G-quadruplex DNA DNA detection Lysozyme detection |
| DOI | 10.1016/j.snb.2018.03.039 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; China Postdoctoral Science Foundation[2016 M602199] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-ES02] |
| WOS Research Area | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS Subject | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| WOS ID | WOS:000430232500057 |
| Publisher | ELSEVIER SCIENCE SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158939 |
| Collection | 海洋环境腐蚀与与生物污损重点实验室 |
| Corresponding Author | Zhang, Dun; Qi, Peng |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zeng, Yan,Zhang, Dun,Qi, Peng,et al. Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,265:468-475. |
| APA | Zeng, Yan,Zhang, Dun,Qi, Peng,&Zheng, Laibao.(2018).Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure.SENSORS AND ACTUATORS B-CHEMICAL,265,468-475. |
| MLA | Zeng, Yan,et al."Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure".SENSORS AND ACTUATORS B-CHEMICAL 265(2018):468-475. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Label-free simultane（1787KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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