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First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF
Gu, Han-jie1,2,3,4; Sun, Qing-lei1,2,3; Jiang, Shuai1,2,3; Zhang, Jian1,2,3,5; Sun, Li1,2,3
2018-07-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume84Pages:382-395
Corresponding AuthorZhang, Jian(zhangjian@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
AbstractAnti-lipopolysaccharide factor (ALF) is a type of antimicrobial peptides (AMPs) with a vital role in antimicrobial defense. Although a large amount of ALFs have been identified from neritic and fresh water crustacean species, no functional investigation of ALFs from deep-sea animals have been documented. In the present study, we characterized the immune function of an ALF molecule (named RspALF1) from the shrimp Rimicaris sp. residing in the deep-sea hydrothermal vent in Desmos, Manus Basin. RspALF1 shares 51.5%-62.4% overall sequence identities with known shrimp ALFs and contains the conserved LPS binding domain (LBD). Both recombinant RspALF1 (rRspALF1) and the LBD-derived peptide (ALF1P1) bound to the cell wall components of Gram-negative and Gram-positive bacteria and killed a wide range of bacteria, especially those from deep-sea hydrothermal field, by damaging bacterial cellular structures. The bactericidal activities of rRspALF1 and ALF1P1 were optimal and stably maintained from 4 C to 37 C, which is comparable to the ambient temperature range of the habitat of Rimicaris sp. In addition to bacteria, rRspALF1 and ALF1P1 also exhibited anti-fungal activity. rRspALF1 and ALF1P1 exhibited high killing efficiencies, which, in terms of MIC values, were ranged between 0.25 M and 4 M for bacteria and 4 mu M-81.1 mu M for fungi. When introduced in vivo, both rRspALF1 and ALF1P1 effectively inhibited bacterial infection in shrimp and reduced the dissemination of bacterial and viral pathogens in fish. Together, these results provide the first insight into the biological property of deep-sea ALF and indicate that RspALF1 very likely plays a significant role in immune defense by functioning as a highly effective antimicrobial with a broad target range. (C) 2018 Elsevier Ltd. All rights reserved.
KeywordAnti-lipopolysaccharide factor Antimicrobial peptides Rimicaris sp. Deep-sea hydrothermal vent Antibacterial Antiviral
DOI10.1016/j.dci.2018.03.016
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of Chinese Academy of Sciences[XDA11030401] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2016ASKJ14] ; AoShan Talents Program by Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000431160800037
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158927
Collection实验海洋生物学重点实验室
深海极端环境与生命过程研究中心
Corresponding AuthorZhang, Jian; Sun, Li
Affiliation1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Gu, Han-jie,Sun, Qing-lei,Jiang, Shuai,et al. First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,84:382-395.
APA Gu, Han-jie,Sun, Qing-lei,Jiang, Shuai,Zhang, Jian,&Sun, Li.(2018).First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,84,382-395.
MLA Gu, Han-jie,et al."First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 84(2018):382-395.
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