Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia | |
Han, Si-yin1,2,3 ; Wang, Meng-qiang1,2; Liu, Mei1,2; Wang, Bao-jie1,2; Jiang, Ke-yong1,2; Wang, Lei1,2
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| 2018-03-01 | |
| Source Publication | AQUACULTURE
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| ISSN | 0044-8486 |
| Volume | 490Pages:44-52 |
| Corresponding Author | Wang, Lei(wanglei@qdio.ac.cn) |
| Abstract | Low dissolved oxygen (DO) could cause a stress response in shrimp, the objective of this study was to compare sensitivity of the hepatopancreas and midgut to oxidative stress under low DO. The white shrimp Litopenaeus vannamei were reared under conditions of cyclic serious/medium hypoxia (CSMH, 0.8-3.5 mg/L) versus normoxia (N, 6.4-7.5 mg/L) for a 28-day period. Survival and growth performance of shrimp were evaluated, and hypoxia inducible factors 1a (HIF-1 alpha) expression, antioxidant responses, apoptosis and histology in the hepatopancreas and midgut were investigated. Antioxidant responses tested included the following: manganese superoxide dismutase, glutathione peroxidases, glutathione-S-transferase, metallothionein and heat shock protein70 expression. Results showed enhanced HIF-1 alpha expression and antioxidant responses in the hepatopancreas and midgut during short-term cyclic serious/medium hypoxia (<= 7 days), which suggested early adaptive mechanism of shrimp to tolerate low DO and avoid oxidative damage. Meanwhile, HIF-1 alpha expression, apoptosis and histopathological lesions were induced earlier in the hepatopancreas than the midgut. Thus, the hepatopancreas could be more sensitive to low DO and its oxidative stress than the midgut. However, long-term (>= 14 days) cyclic serious/medium hypoxia could disrupt cellular antioxidant mechanism with depressed antioxidant responses, and then aggravate apoptosis and histopathological lesions in the hepatopancreas and midgut, particularly the hepatopancreas would lose antioxidant ability earlier than the midgut because of higher sensitivity to oxidative stress. Therefore, we have a few insights that it is vital to protect hepatopancreas for controlling shrimp death and growth inhibition under cyclic serious/medium hypoxia. |
| Keyword | Litopenaeus vannamei Hepatopancreas Midgut Cyclic serious/medium hypoxia Oxidative stress Gene expression |
| DOI | 10.1016/j.aquaculture.2018.02.021 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | STS Key Deployment Project of Chinese Academy of Sciences[KFZD-SW-106] |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000429323600007 |
| Publisher | ELSEVIER SCIENCE BV |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158803 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Wang, Lei |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Han, Si-yin,Wang, Meng-qiang,Liu, Mei,et al. Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia[J]. AQUACULTURE,2018,490:44-52. |
| APA | Han, Si-yin,Wang, Meng-qiang,Liu, Mei,Wang, Bao-jie,Jiang, Ke-yong,&Wang, Lei.(2018).Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia.AQUACULTURE,490,44-52. |
| MLA | Han, Si-yin,et al."Comparative sensitivity of the hepatopancreas and midgut in the white shrimp Litopenaeus vannamei to oxidative stress under cyclic serious/medium hypoxia".AQUACULTURE 490(2018):44-52. |
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| Comparative sensitiv（621KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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