Involvement of p90 ribosomal S6 kinase in termination of cell cycle arrest during development of Artemia-encysted embryos

doi:10.1074/jbc.M707853200

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	Involvement of p90 ribosomal S6 kinase in termination of cell cycle arrest during development of Artemia-encysted embryos
	Dai, Jie-Qiong 1; Zhu, Xiao-Jing 1; Liu, Feng-Qi 2; Xiang, Jian-Hai3 ; Nagasawa, Hiromichi 4; Yang, Wei-Jun 1; Yang, WJ, Zhejiang Univ, Inst Cell Biol & Genet, Coll Life Sci, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China
	2008-01-18
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN	0021-9258
卷号	283 期号:3 页码:1705-1712
文章类型	Article
摘要	Artemia has evolved a unique developmental pattern of encysted embryos to cope with various environmental threats. Cell divisions totally cease during the preemergence developmental stage from gastrula to prenauplius. The molecular mechanism of this, however, remains unknown. Our study focuses on the involvement of p90 ribosomal S6 kinase (RSK), a family of serine/threonine kinase-mediating signal transduction downstream of mitogen-activated protein kinase cascades, in the termination of cell cycle arrest during the post-embryonic development of Artemia-encysted gastrula. With immunochemistry, morphology, and cell cycle analysis, the identified Artemia RSK was established to be specifically activated during the post-embryonic and early larval developmental stages when arrested cells of encysted embryos resumed mitoses. In vivo knockdown of RSK activity by RNA interference, kinase inhibition, and antibody neutralization consistently induced defective larvae with distinct gaps between the exoskeleton and internal tissues. In these abnormal individuals, mitoses were detected to be largely inhibited in the affected regions. These results display the requirement of RSK activity during Artemia development and suggest its role in termination of cell cycle (G(2)/M phase) arrest and promotion of mitogenesis. Our findings may, thus, provide insights into the regulation of cell division during Artemia post-embryonic development and reveal further aspects of RSK functions.; Artemia has evolved a unique developmental pattern of encysted embryos to cope with various environmental threats. Cell divisions totally cease during the preemergence developmental stage from gastrula to prenauplius. The molecular mechanism of this, however, remains unknown. Our study focuses on the involvement of p90 ribosomal S6 kinase (RSK), a family of serine/threonine kinase-mediating signal transduction downstream of mitogen-activated protein kinase cascades, in the termination of cell cycle arrest during the post-embryonic development of Artemia-encysted gastrula. With immunochemistry, morphology, and cell cycle analysis, the identified Artemia RSK was established to be specifically activated during the post-embryonic and early larval developmental stages when arrested cells of encysted embryos resumed mitoses. In vivo knockdown of RSK activity by RNA interference, kinase inhibition, and antibody neutralization consistently induced defective larvae with distinct gaps between the exoskeleton and internal tissues. In these abnormal individuals, mitoses were detected to be largely inhibited in the affected regions. These results display the requirement of RSK activity during Artemia development and suggest its role in termination of cell cycle (G(2)/M phase) arrest and promotion of mitogenesis. Our findings may, thus, provide insights into the regulation of cell division during Artemia post-embryonic development and reveal further aspects of RSK functions.
关键词	Activated Protein-kinase Map Kinase P90(Rsk) Rsk Phosphorylation Domains Identification Proliferation Replication Salina
学科领域	Biochemistry & Molecular Biology
DOI	10.1074/jbc.M707853200
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000252282700057
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1587
专题	实验海洋生物学重点实验室
通讯作者	Yang, WJ, Zhejiang Univ, Inst Cell Biol & Genet, Coll Life Sci, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China
作者单位	1.Zhejiang Univ, Inst Cell Biol & Genet, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China 2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 4.Univ Tokyo, Dept Appl Biol Chem, Grad Sch Agr & Life Sci, Tokyo 1138657, Japan
推荐引用方式 GB/T 7714	Dai, Jie-Qiong,Zhu, Xiao-Jing,Liu, Feng-Qi,et al. Involvement of p90 ribosomal S6 kinase in termination of cell cycle arrest during development of Artemia-encysted embryos[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2008,283(3):1705-1712.
APA	Dai, Jie-Qiong.,Zhu, Xiao-Jing.,Liu, Feng-Qi.,Xiang, Jian-Hai.,Nagasawa, Hiromichi.,...&Yang, WJ, Zhejiang Univ, Inst Cell Biol & Genet, Coll Life Sci, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China.(2008).Involvement of p90 ribosomal S6 kinase in termination of cell cycle arrest during development of Artemia-encysted embryos.JOURNAL OF BIOLOGICAL CHEMISTRY,283(3),1705-1712.
MLA	Dai, Jie-Qiong,et al."Involvement of p90 ribosomal S6 kinase in termination of cell cycle arrest during development of Artemia-encysted embryos".JOURNAL OF BIOLOGICAL CHEMISTRY 283.3(2008):1705-1712.

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