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Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China
Fu, Songzhe1; Wang, Liping2; Tian, Huiqin3; Wei, Dawei4; Liu, Ying1
2018-04-01
Source PublicationANNALS OF MICROBIOLOGY
ISSN1590-4261
Volume68Issue:4Pages:175-184
Corresponding AuthorFu, Songzhe(fusongzhe@hotmail.com)
AbstractAcute hepatopancreatic necrosis disease (AHPND) outbreaks in cultured shrimps were identified in Zhangpu, China. One Vibrio parahaemolyticus strain PB1937 was isolated from the cultured shrimps and was confirmed as a causative agent of the AHPND outbreak by employing Koch's four postulates. Challenge tests with 10(6) cells ml(-1) of strain PB1937 caused 100% mortality of shrimps, indicating it had sufficient virulence to cause the outbreak. Phylogenomic analysis revealed a clear divergence between PB1937 and 14 publicly available V. parahaemolyticus strains and divided 11 AHPND-causing strains into six genomic clusters. Prophage profiling of above strains revealed strong correlations with their genomic relationship, while Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) were almost absent in the genomes. The binary toxin gene pirAB(vp) directly related to the development of AHPND was found in a 70-kb plasmid p1937-1 in PB1937 but was absent in a 78-kb novel plasmid p1937-2, which shared 46% sequence similarity with p1937-1. Comparative genomic analysis revealed that PB1937 has a novel truncated type VI secretion system (T6SS1) which possibly affects its antibacterial activity. In addition, three novel genomic islands were reported. The analysis of the genomes gave some clues regarding the correlation of virulence with its genomic trait for the AHPND strains.
KeywordVibrio parahaemolyticus AHPND PacBio pirAB(vp) Prophage Type VI secretion system
DOI10.1007/s13213-018-1328-0
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFD0701700] ; National Natural Science Foundation of China[31672673] ; National Natural Science Foundation of China[31472312]
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000429125500002
PublisherSPRINGER
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158774
Collection中国科学院海洋研究所
Corresponding AuthorFu, Songzhe
Affiliation1.Dalian Ocean Univ, Coll Marine Technol & Environm, 52 Heishijiao St, Dalian 116023, Peoples R China
2.State Oceanog Adm China, Inst Oceanog 3, Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
4.Northwest Agr & Forestry Univ, Coll Life Sci, Yangling 712100, Peoples R China
Recommended Citation
GB/T 7714
Fu, Songzhe,Wang, Liping,Tian, Huiqin,et al. Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China[J]. ANNALS OF MICROBIOLOGY,2018,68(4):175-184.
APA Fu, Songzhe,Wang, Liping,Tian, Huiqin,Wei, Dawei,&Liu, Ying.(2018).Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China.ANNALS OF MICROBIOLOGY,68(4),175-184.
MLA Fu, Songzhe,et al."Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China".ANNALS OF MICROBIOLOGY 68.4(2018):175-184.
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