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Bacterial communities associated with Shinkaia crosnieri from the Iheya North, Okinawa Trough: Microbial diversity and metabolic potentials
Zhang, Jian1,2; Zeng, Zhi-gang3; Chen, Shuai3; Sun, Li1,2
2018-04-01
Source PublicationJOURNAL OF MARINE SYSTEMS
ISSN0924-7963
Volume180Pages:228-236
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractShinkaia crosnieri is a galatheid crab endemic to the deep-sea hydrothermal systems in the Okinawa Trough. In this study, we systematically analyzed and compared the diversity and metabolic potentials of the microbial communities in different tissues (setae, gill, and intestine) of S. crosnieri by high-throughput sequencing technology and quantitative real-time polymerase chain reaction. Sequence analysis based on the V3-V4 regions of the 16S rRNA gene obtained 408,079 taxon tags, which covered 15 phyla, 22 classes, 32 orders, 42 families, and 25 genera. Overall, the microbial communities in all tissues were dominated by Epsilonproteobacteria and Gammaproteobacteria, of which Epsilonproteobacteria was the largest class and accounted for 85.24% of the taxon tags. In addition, 20 classes of bacteria were discovered for the first time to be associated with S. crosnieri and no archaea were detected. Comparative analysis showed that (i) bacteria from different tissues fell into different groups by beta-diversity analysis, (ii) bacterial communities in intestine were similar to that in gill and much more diverse than that in setae, and the sulfur-oxidizing genus Sulfurovum was markedly enriched in intestine and gill. Furthermore, bacteria potentially involved in methane, nitrogen, and metal metabolisms were detected in all samples. The key genes of aprA/dsrA and pmoA involved in sulfate reducing and methane oxidization, respectively, were detected in the gill and gut communities for the first time, and pmoA was significantly more abundant in gill and setae than in intestine. These results provide the first comparative and relatively complete picture of the diversity and metabolic potentials of the bacteria in different tissues of S. crosnieri. These results also indicate that the composition of the microbial communities in hydrothermal fauna changes with time, suggesting the importance of environmental influence. (C) 2017 Elsevier B.V. All rights reserved.
KeywordShinkaia crosnieri 165 rRNA gene Microbial community Metabolism Sulfur oxidization
DOI10.1016/j.jmarsys.2017.02.011
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA11030401] ; Strategic Biological Resources Service Network Plan of Chinese Academy of Sciences[ZSSD-005] ; National Basic Research Program of China[2013CB429700] ; Taishan Scholar Program of Shandong Province
WOS Research AreaGeology ; Marine & Freshwater Biology ; Oceanography
WOS SubjectGeosciences, Multidisciplinary ; Marine & Freshwater Biology ; Oceanography
WOS IDWOS:000429514700022
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158771
Collection实验海洋生物学重点实验室
海洋地质与环境重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Jian,Zeng, Zhi-gang,Chen, Shuai,et al. Bacterial communities associated with Shinkaia crosnieri from the Iheya North, Okinawa Trough: Microbial diversity and metabolic potentials[J]. JOURNAL OF MARINE SYSTEMS,2018,180:228-236.
APA Zhang, Jian,Zeng, Zhi-gang,Chen, Shuai,&Sun, Li.(2018).Bacterial communities associated with Shinkaia crosnieri from the Iheya North, Okinawa Trough: Microbial diversity and metabolic potentials.JOURNAL OF MARINE SYSTEMS,180,228-236.
MLA Zhang, Jian,et al."Bacterial communities associated with Shinkaia crosnieri from the Iheya North, Okinawa Trough: Microbial diversity and metabolic potentials".JOURNAL OF MARINE SYSTEMS 180(2018):228-236.
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