IOCAS-IR  > 海洋环境腐蚀与与生物污损重点实验室
A simple, rapid and cost-effective colorimetric assay based on the 4-mercaptophenylboronic acid functionalized silver nanoparticles for bacteria monitoring
Zheng, Laibao1,2,3; Qi, Peng1,3; Zhang, Dun1,3
2018-05-01
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume260Pages:983-989
Corresponding AuthorQi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
AbstractThe rapid and cost-effective detection of bacteria is of great importance in various fields. Herein, a novel colorimetric detection method for Gram-negative bacteria based on the inhibition of the aggregation of 4-mercaptophenylboronic acid functionalized AgNPs (MPBA-AgNPs) was demonstrated. MPBA, one of the boronic acid derivative, which could react with the cis-diol groups contained in saccharides on the bacteria cells, was used as the recognition molecule for bacteria detection. The aggregation of MPBA-AgNPs could be inhibited by bacterial cells, resulting in different color change, which could be distinguished with UV-vis spectrometer and the naked eye. The concentration of E. coli, a model of Gram-negative bacteria, could be determined in a dynamic range from 5 x 10(4) cfu mL(-1) to 1 x 10(7) cfu mL(-1) with a LOD of 0.9 x 10(4) cfu mL(-1) with fast response of 20 min. Meanwhile, a smartphone based measuring analysis was established to make it suitable for routine laboratory applications. Moreover, the concept of the MPBA-AgNPs based colorimetric strategy could be expanded to other Gram-negative bacteria suggesting a universal platform for bacterial assay. (C) 2018 Elsevier B.V. All rights reserved.
KeywordSilver nanoparticles Colorimetric assay Bacteria detection 4-Mercaptophenylboronic acid Smartphone
DOI10.1016/j.snb.2018.01.115
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; Science & Technology Basic Research Program of Qingdao[16-5-1-19-jch] ; AoShan Talent Program ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-ES02]
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000424884300115
PublisherELSEVIER SCIENCE SA
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158762
Collection海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorQi, Peng; Zhang, Dun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zheng, Laibao,Qi, Peng,Zhang, Dun. A simple, rapid and cost-effective colorimetric assay based on the 4-mercaptophenylboronic acid functionalized silver nanoparticles for bacteria monitoring[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,260:983-989.
APA Zheng, Laibao,Qi, Peng,&Zhang, Dun.(2018).A simple, rapid and cost-effective colorimetric assay based on the 4-mercaptophenylboronic acid functionalized silver nanoparticles for bacteria monitoring.SENSORS AND ACTUATORS B-CHEMICAL,260,983-989.
MLA Zheng, Laibao,et al."A simple, rapid and cost-effective colorimetric assay based on the 4-mercaptophenylboronic acid functionalized silver nanoparticles for bacteria monitoring".SENSORS AND ACTUATORS B-CHEMICAL 260(2018):983-989.
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