Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda

doi:10.1016/j.fsi.2018.02.004

IOCAS-IR > 实验海洋生物学重点实验室

	Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda
	Sun, Yuying 1; Zhang, Jiquan 1,2,3; Xiang, Jianhai2,3
	2018-04-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	75 页码:115-123
通讯作者	Zhang, Jiquan(zhangjiquan@qdio.ac.cn)
摘要	Chitin deacetylase (CDA, EC 3.5.1.41), belonging to a family of extracellular chitin-modifying enzymes, can catalyze the deacetylation of chitin. In this study, the full-length cDNA sequence encoding chitin deacetylase 1 (EcCDA1) was obtained from Exopalaemon carinicauda. The complete nucleotide sequence of EcCDA1 contained a 1611 bp open reading frame (ORF) encoding EcCDA1 precursor of 536 amino acids. The domain architecture of the deduced EcCDA1 protein contained a signal peptide, a chitin-binding peritrophin-A domain (ChtBD2), a low density lipoprotein receptor class A domain (LDLa) and a Polysacc_deac_1 domain. EcCDA1 mRNA was predominantly expressed in the gills. The expression of EcCDA1 in the prawns challenged with Vibrio parahaemolyticus and Aeromonas hydrophila changed in a time-dependent manner. The expression of EcCDA1 in the prawns challenged with V. parahaemolyticus was up-regulated at 12 h (p < 0.05), and significantly up-regulated at 24 h and 48 h (p < 0.01), and then returned to the control levels at 96 h post-challenge (p > 0.05). At the same time, the expression in Aeromonas-challenged group was significantly up-regulated at 12, 24 and 48 h (p < 0.01) and returned to the control levels at 120h post-challenge (p > 0.05). Then, EcCDA1 was recombinantly expressed in Pichia pastoris and the purified recombinant EcCDA1 could not inhibit the growth of V. parahaemolyticus or A. hydrophila, which indicated that the CDA1 may play its biological activity in immune defense by deacetylation from chitin.
关键词	Exopalaemon carinicauda Chitin deacetylase Pichia pastoris
DOI	10.1016/j.fsi.2018.02.004
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31172449] ; National Natural Science Foundation of China[41376165] ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; National High Technology Research and Development Program of China[2012AA10A401] ; National Natural Science Foundation of China[31172449] ; National Natural Science Foundation of China[41376165] ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; National High Technology Research and Development Program of China[2012AA10A401]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000428824900014
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158741
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Jiquan
作者单位	1.Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Yuying,Zhang, Jiquan,Xiang, Jianhai. Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda[J]. FISH & SHELLFISH IMMUNOLOGY,2018,75:115-123.
APA	Sun, Yuying,Zhang, Jiquan,&Xiang, Jianhai.(2018).Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda.FISH & SHELLFISH IMMUNOLOGY,75,115-123.
MLA	Sun, Yuying,et al."Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda".FISH & SHELLFISH IMMUNOLOGY 75(2018):115-123.

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