Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda | |
Sun, Yuying1; Zhang, Jiquan1,2,3; Xiang, Jianhai2,3
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| 2018-04-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| Volume | 75Pages:115-123 |
| Corresponding Author | Zhang, Jiquan(zhangjiquan@qdio.ac.cn) |
| Abstract | Chitin deacetylase (CDA, EC 3.5.1.41), belonging to a family of extracellular chitin-modifying enzymes, can catalyze the deacetylation of chitin. In this study, the full-length cDNA sequence encoding chitin deacetylase 1 (EcCDA1) was obtained from Exopalaemon carinicauda. The complete nucleotide sequence of EcCDA1 contained a 1611 bp open reading frame (ORF) encoding EcCDA1 precursor of 536 amino acids. The domain architecture of the deduced EcCDA1 protein contained a signal peptide, a chitin-binding peritrophin-A domain (ChtBD2), a low density lipoprotein receptor class A domain (LDLa) and a Polysacc_deac_1 domain. EcCDA1 mRNA was predominantly expressed in the gills. The expression of EcCDA1 in the prawns challenged with Vibrio parahaemolyticus and Aeromonas hydrophila changed in a time-dependent manner. The expression of EcCDA1 in the prawns challenged with V. parahaemolyticus was up-regulated at 12 h (p < 0.05), and significantly up-regulated at 24 h and 48 h (p < 0.01), and then returned to the control levels at 96 h post-challenge (p > 0.05). At the same time, the expression in Aeromonas-challenged group was significantly up-regulated at 12, 24 and 48 h (p < 0.01) and returned to the control levels at 120h post-challenge (p > 0.05). Then, EcCDA1 was recombinantly expressed in Pichia pastoris and the purified recombinant EcCDA1 could not inhibit the growth of V. parahaemolyticus or A. hydrophila, which indicated that the CDA1 may play its biological activity in immune defense by deacetylation from chitin. |
| Keyword | Exopalaemon carinicauda Chitin deacetylase Pichia pastoris |
| DOI | 10.1016/j.fsi.2018.02.004 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31172449] ; National Natural Science Foundation of China[41376165] ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; National High Technology Research and Development Program of China[2012AA10A401] |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000428824900014 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158741 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Zhang, Jiquan |
| Affiliation | 1.Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| Corresponding Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Sun, Yuying,Zhang, Jiquan,Xiang, Jianhai. Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda[J]. FISH & SHELLFISH IMMUNOLOGY,2018,75:115-123. |
| APA | Sun, Yuying,Zhang, Jiquan,&Xiang, Jianhai.(2018).Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda.FISH & SHELLFISH IMMUNOLOGY,75,115-123. |
| MLA | Sun, Yuying,et al."Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda".FISH & SHELLFISH IMMUNOLOGY 75(2018):115-123. |
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