Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection | |
Du, Yishuai1; Liu, Pengfei1,2; Meng, Lingjie1,2 ; Sharawy, Zaki3; Liu, Ying4
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| 2018-05-01 | |
| Source Publication | AQUACULTURE RESEARCH
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| ISSN | 1355-557X |
| Volume | 49Issue:5Pages:1826-1833 |
| Corresponding Author | Liu, Ying(yingliu@dlou.edu.cn) |
| Abstract | Aeromonassalmonicida subsp. masoucida (ASM) is classified as atypical A.salmonicida and brought huge economic damages to the local salmonid aquaculture in China. An ASM strain named AS-C4 was used to investigate the colonization of ASM in Atlantic salmon (Salmo salarL.) by an immersion challenge with the control group (T0, no AS-C4), group T1 (2.67x10(4) CFU/ml AS-C4) and group T2 (2.67x10(7) CFU/ml AS-C4). The numbers of AS-C4 copies in different fish tissues (gill, intestine, skin, blood, muscle, spleen, liver and kidney) were determined at different time points post challenge using the quantitative real-time PCR (qRT-PCR). AS-C4 were detected in the gill and intestine as early as 0hr after the challenge both in T1 and T2 groups, suggesting that the gill and intestine were probably the portals of entry of AS-C4 into salmon. Although AS-C4 could not be detected in the skin until 24hr after the challenge in T1 group, it could be detected in the skin as early as 0hr after the challenge in T2 group, indicating that the skin may also be a portal of entry of AS-C4 into salmon. AS-C4 was immediately detected in the blood within 3hr after it entered the host, suggesting that AS-C4 successfully invaded the bloodstream of fish. After AS-C4 colonized the host, it colonized the internal tissues, such as the spleen, liver, kidney and muscle. The results of this study will contribute to the understanding of the pathogenesis of the ASM strains and give a broader understanding of the infection route of ASM in it's host, providing more information for the development of new therapeutic strategies to protect against this pathogen in aquaculture. |
| Keyword | Aeromonassalmonicida Atlantic salmon colonization immersion infection route |
| DOI | 10.1111/are.13637 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2017YFD0701700] ; 56th China Postdoctoral Science Foundation[2014M560580] ; Postdoctoral Innovation Project Special Funds of Shandong Province[201402005] ; Qingdao Innovation Talents Program[13-CX-16] |
| WOS Research Area | Fisheries |
| WOS Subject | Fisheries |
| WOS ID | WOS:000429410800009 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158672 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu, Ying |
| Affiliation | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Earth & Environm Sci Coll, Beijing, Peoples R China 3.Natl Inst Oceanog & Fisheries NIOF, Aquaculture Div, Suez, Egypt 4.Dalian Ocean Univ, Marine Sci & Environm Engn Sch, Dalian, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Du, Yishuai,Liu, Pengfei,Meng, Lingjie,et al. Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection[J]. AQUACULTURE RESEARCH,2018,49(5):1826-1833. |
| APA | Du, Yishuai,Liu, Pengfei,Meng, Lingjie,Sharawy, Zaki,&Liu, Ying.(2018).Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection.AQUACULTURE RESEARCH,49(5),1826-1833. |
| MLA | Du, Yishuai,et al."Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection".AQUACULTURE RESEARCH 49.5(2018):1826-1833. |
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| Colonization of Aero（468KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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