Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection

doi:10.1111/are.13637

IOCAS-IR > 实验海洋生物学重点实验室

	Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection
	Du, Yishuai 1; Liu, Pengfei 1,2; Meng, Lingjie1,2 ; Sharawy, Zaki 3; Liu, Ying4
	2018-05-01
发表期刊	AQUACULTURE RESEARCH
ISSN	1355-557X
卷号	49 期号:5 页码:1826-1833
通讯作者	Liu, Ying(yingliu@dlou.edu.cn)
摘要	Aeromonassalmonicida subsp. masoucida (ASM) is classified as atypical A.salmonicida and brought huge economic damages to the local salmonid aquaculture in China. An ASM strain named AS-C4 was used to investigate the colonization of ASM in Atlantic salmon (Salmo salarL.) by an immersion challenge with the control group (T0, no AS-C4), group T1 (2.67x10(4) CFU/ml AS-C4) and group T2 (2.67x10(7) CFU/ml AS-C4). The numbers of AS-C4 copies in different fish tissues (gill, intestine, skin, blood, muscle, spleen, liver and kidney) were determined at different time points post challenge using the quantitative real-time PCR (qRT-PCR). AS-C4 were detected in the gill and intestine as early as 0hr after the challenge both in T1 and T2 groups, suggesting that the gill and intestine were probably the portals of entry of AS-C4 into salmon. Although AS-C4 could not be detected in the skin until 24hr after the challenge in T1 group, it could be detected in the skin as early as 0hr after the challenge in T2 group, indicating that the skin may also be a portal of entry of AS-C4 into salmon. AS-C4 was immediately detected in the blood within 3hr after it entered the host, suggesting that AS-C4 successfully invaded the bloodstream of fish. After AS-C4 colonized the host, it colonized the internal tissues, such as the spleen, liver, kidney and muscle. The results of this study will contribute to the understanding of the pathogenesis of the ASM strains and give a broader understanding of the infection route of ASM in it's host, providing more information for the development of new therapeutic strategies to protect against this pathogen in aquaculture.
关键词	Aeromonassalmonicida Atlantic salmon colonization immersion infection route
DOI	10.1111/are.13637
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2017YFD0701700] ; 56th China Postdoctoral Science Foundation[2014M560580] ; Postdoctoral Innovation Project Special Funds of Shandong Province[201402005] ; Qingdao Innovation Talents Program[13-CX-16] ; National Key R&D Program of China[2017YFD0701700] ; 56th China Postdoctoral Science Foundation[2014M560580] ; Postdoctoral Innovation Project Special Funds of Shandong Province[201402005] ; Qingdao Innovation Talents Program[13-CX-16]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:000429410800009
出版者	WILEY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158672
专题	实验海洋生物学重点实验室
通讯作者	Liu, Ying
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Earth & Environm Sci Coll, Beijing, Peoples R China 3.Natl Inst Oceanog & Fisheries NIOF, Aquaculture Div, Suez, Egypt 4.Dalian Ocean Univ, Marine Sci & Environm Engn Sch, Dalian, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Du, Yishuai,Liu, Pengfei,Meng, Lingjie,et al. Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection[J]. AQUACULTURE RESEARCH,2018,49(5):1826-1833.
APA	Du, Yishuai,Liu, Pengfei,Meng, Lingjie,Sharawy, Zaki,&Liu, Ying.(2018).Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection.AQUACULTURE RESEARCH,49(5),1826-1833.
MLA	Du, Yishuai,et al."Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection".AQUACULTURE RESEARCH 49.5(2018):1826-1833.

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