IOCAS-IR  > 实验海洋生物学重点实验室
Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection
Du, Yishuai1; Liu, Pengfei1,2; Meng, Lingjie1,2; Sharawy, Zaki3; Liu, Ying4
2018-05-01
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume49Issue:5Pages:1826-1833
Corresponding AuthorLiu, Ying(yingliu@dlou.edu.cn)
AbstractAeromonassalmonicida subsp. masoucida (ASM) is classified as atypical A.salmonicida and brought huge economic damages to the local salmonid aquaculture in China. An ASM strain named AS-C4 was used to investigate the colonization of ASM in Atlantic salmon (Salmo salarL.) by an immersion challenge with the control group (T0, no AS-C4), group T1 (2.67x10(4) CFU/ml AS-C4) and group T2 (2.67x10(7) CFU/ml AS-C4). The numbers of AS-C4 copies in different fish tissues (gill, intestine, skin, blood, muscle, spleen, liver and kidney) were determined at different time points post challenge using the quantitative real-time PCR (qRT-PCR). AS-C4 were detected in the gill and intestine as early as 0hr after the challenge both in T1 and T2 groups, suggesting that the gill and intestine were probably the portals of entry of AS-C4 into salmon. Although AS-C4 could not be detected in the skin until 24hr after the challenge in T1 group, it could be detected in the skin as early as 0hr after the challenge in T2 group, indicating that the skin may also be a portal of entry of AS-C4 into salmon. AS-C4 was immediately detected in the blood within 3hr after it entered the host, suggesting that AS-C4 successfully invaded the bloodstream of fish. After AS-C4 colonized the host, it colonized the internal tissues, such as the spleen, liver, kidney and muscle. The results of this study will contribute to the understanding of the pathogenesis of the ASM strains and give a broader understanding of the infection route of ASM in it's host, providing more information for the development of new therapeutic strategies to protect against this pathogen in aquaculture.
KeywordAeromonassalmonicida Atlantic salmon colonization immersion infection route
DOI10.1111/are.13637
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFD0701700] ; 56th China Postdoctoral Science Foundation[2014M560580] ; Postdoctoral Innovation Project Special Funds of Shandong Province[201402005] ; Qingdao Innovation Talents Program[13-CX-16]
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000429410800009
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158672
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Ying
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Earth & Environm Sci Coll, Beijing, Peoples R China
3.Natl Inst Oceanog & Fisheries NIOF, Aquaculture Div, Suez, Egypt
4.Dalian Ocean Univ, Marine Sci & Environm Engn Sch, Dalian, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Du, Yishuai,Liu, Pengfei,Meng, Lingjie,et al. Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection[J]. AQUACULTURE RESEARCH,2018,49(5):1826-1833.
APA Du, Yishuai,Liu, Pengfei,Meng, Lingjie,Sharawy, Zaki,&Liu, Ying.(2018).Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection.AQUACULTURE RESEARCH,49(5),1826-1833.
MLA Du, Yishuai,et al."Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection".AQUACULTURE RESEARCH 49.5(2018):1826-1833.
Files in This Item:
File Name/Size DocType Version Access License
Colonization of Aero(468KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Du, Yishuai]'s Articles
[Liu, Pengfei]'s Articles
[Meng, Lingjie]'s Articles
Baidu academic
Similar articles in Baidu academic
[Du, Yishuai]'s Articles
[Liu, Pengfei]'s Articles
[Meng, Lingjie]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Du, Yishuai]'s Articles
[Liu, Pengfei]'s Articles
[Meng, Lingjie]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.