Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Germ Cell Migration, Proliferation and Differentiation during Gonadal Morphogenesis in All-Female Japanese Flounder (Paralichthys Olivaceus) | |
Yang, Yang1,2,3 ; Liu, Qinghua1,2; Xiao, Yongshuang1,2; Wang, Xueying1,3; An, Hao1,2,3; Song, Zongcheng4; You, Feng1,2,3; Wang, Yanfeng1,3; Ma, Daoyuan1,3; Li, Jun1,3
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| 2018-04-01 | |
| Source Publication | ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
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| ISSN | 1932-8486 |
| Volume | 301Issue:4Pages:727-741 |
| Corresponding Author | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| Abstract | In this study, all genetic female (XX) broods of Japanese flounder were produced artificially by mating the females with sex-reversed males. The proliferation and migration of primordial germ cells (PGCs), formation of ovary and oogenesis were described in detail. After hatching, around 20 individual PGCs migrated from the lateral to the dorsal of trunk region. At 15 days posthatching (dph), a part of PGCs were covered by a single layer somatic cells and formed the genital ridge. By 22 dph, the elongated gonadal primordia appeared under the ventral kidney, where the PGCs were totally enclosed by somatic cells. During the process of migration, PGCs were presumed to be mitotically inactive. From 63 to 73 dph, somatic cells rearrangement resulted in the formation of a narrow crevice, which became deeper and formed ovarian lumen. However, at 52 dph, dramatic mitotic proliferation of germ cell occurred and germline nest formed before the appearance of ovarian lumen. The onset of intensive germ cell proliferation and appearance of cell nests could be accepted as a criterion of initial ovarian differentiation. Then germ cells and somatic epithelial cells were gradually delimited by basement membrane and formed the germinal epithelium. In this period, results from in situ hybridization revealed that the early forkhead box L2 (pofoxl2) was expressed in somatic cells and oocytes in primary growth, which indicated the prefollicle cells formed. Then oogonia or oocytes, follicle cells, basement membrane, and theca cells composed a follicle complex. Finally, oocytes underwent meiosis and developed into to mature eggs. Anat Rec, 301:727-741, 2018. (c) 2017 Wiley Periodicals, Inc. |
| Keyword | Japanese flounder migration PGCs oogenesis foxl2 |
| DOI | 10.1002/ar.23698 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31372514] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; Modern Agro-Industry Technology Research System[nycytx-50] ; China Postdoctoral Science Foundation[2014M551973] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of science and technology service network planning[KFJ-EW-STS-060] |
| WOS Research Area | Anatomy & Morphology |
| WOS Subject | Anatomy & Morphology |
| WOS ID | WOS:000427069100014 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158423 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu, Qinghua; Li, Jun |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
| First Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Yang, Yang,Liu, Qinghua,Xiao, Yongshuang,et al. Germ Cell Migration, Proliferation and Differentiation during Gonadal Morphogenesis in All-Female Japanese Flounder (Paralichthys Olivaceus)[J]. ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY,2018,301(4):727-741. |
| APA | Yang, Yang.,Liu, Qinghua.,Xiao, Yongshuang.,Wang, Xueying.,An, Hao.,...&Li, Jun.(2018).Germ Cell Migration, Proliferation and Differentiation during Gonadal Morphogenesis in All-Female Japanese Flounder (Paralichthys Olivaceus).ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY,301(4),727-741. |
| MLA | Yang, Yang,et al."Germ Cell Migration, Proliferation and Differentiation during Gonadal Morphogenesis in All-Female Japanese Flounder (Paralichthys Olivaceus)".ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY 301.4(2018):727-741. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Germ Cell Migration,（2226KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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