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Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis
Jia, Zhihao1,3; Wang, Mengqiang1; Wang, Xiudan1,3; Wang, Lingling2; Qiu, Limei1; Song, Linsheng2,4
2018-05-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume82Pages:94-103
Corresponding AuthorQiu, Limei(qiulimei@qdio.ac.cn) ; Song, Linsheng(lshsong@qdio.ac.cn)
AbstractReactive oxygen species (ROS) produced in vivo during various electron transfer reactions are generally kept at a certain level since they are harmful to cells. However, it can sensitize hematopoietic progenitors to differentiation, and plays a signaling role in the regulation of hematopoietic cell fate. In the present study, the transcriptomes of crab HPT and hemocytes were sequenced using the Ion Torrent Proton sequencing platform. A total of 51,229,690 single end reads were obtained from six single-end libraries, which were assembled into 31346 unireads as reference. After mapping and transcript assembling, 362 differently expressed genes were identified and 301 of them were deemed to be more abundant in HPT. GO annotation revealed that they were mostly implicated in DNA, RNA and protein synthesis, cell division, mitochondria activities and energy metabolism. The expression level of mitochondrial complexes I (mitochondrial NADH-ubiquinone oxidoreductase) which was the main natural producers of mitochondrial ROS was found to be 8.6-fold (p < 0.01) higher in HPT than that in hemocytes. In hemocytes, the proteinase genes associated with proPO activation from the 61 up-regulated genes in hemocytes were the main up-regulated genes which might be the potential markers for mature hemocytes. ROS level in HPT cells was relatively higher which was confirmed with the high expression level of mitochondria related genes identified by transcriptome sequencing. After the ROS level was depressed by N-acetyk-cysteine (NAC), the production of hemocytes from HPT was inhibited, and the recovery of the total hemocytes counts was delayed. These results collectively indicated that the genes in redox system were more active in HPT, and ROS could function as an important modulator in the hematopoiesis of crab and promote the production of hemocytes from HPT. (C) 2018 Elsevier Ltd. All rights reserved.
KeywordEriocheir sinensis Reactive oxygen species (ROS) Hematopoietic tissue Transcriptome sequencing Mitochondrial complexes I N-acetyl-L-cysteine (NAC)
DOI10.1016/j.dci.2017.12.030
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31530069] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian high level talent innovation support program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] ; AoShan Talents Cultivation Program
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000427668700010
PublisherELSEVIER SCI LTD
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158397
Collection实验海洋生物学重点实验室
Corresponding AuthorQiu, Limei; Song, Linsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Zhihao,Wang, Mengqiang,Wang, Xiudan,et al. Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,82:94-103.
APA Jia, Zhihao,Wang, Mengqiang,Wang, Xiudan,Wang, Lingling,Qiu, Limei,&Song, Linsheng.(2018).Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,82,94-103.
MLA Jia, Zhihao,et al."Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 82(2018):94-103.
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