Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers | |
| Xiao, Yongshuang1,2; Guan, Shuguang3; Liu, Qinghua1,2; Liu, Hongjun3; Yu, Daode3; Ma, Daoyuan1,2; Xu, Shihong1,2; Liu, Jing1; Dai, Ming4; Xiao, Zhizhong1,2; Li, Jun1,2 | |
| 2018-03-01 | |
| 发表期刊 | JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM
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| ISSN | 0025-3154 |
| 卷号 | 98期号:2页码:261-267 |
| 通讯作者 | Xiao, Zhizhong(xzz@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 摘要 | Seven-band grouper (Hyporthodus septemfasciatus) is a commercial rocky reef fish in East Asia that has been regarded as a promising species for aquaculture. To investigate the broodstock contributions to offspring for the sustainability of fry production, 62 individuals of H. septemfasciatus from two broodstocks and one offspring population were analysed using fluorescent-AFLP. A total of 602 bands were amplified and 70.10% of them were polymorphic. The numbers of polymorphic loci were 308 (P-broodstock I = 55.50%) and 356 (P-broodstock II = 63.12%) in the two broodstocks, and 294 (P-offspring = 52.88%) in the offspring, respectively. The average values of Shannon diversity index (I) and expected heterozygosity (H) were higher in the broodstock (I-broodstock I = 0.281, I-broodstock II = 0.244, H-broodstock I = 0.185, H-broodstock II = 0.161) than those in the offspring (I-offspring = 0.243, H-offspring = 0.161). AMOVA and F-ST analyses showed that significant genetic differentiation between broodstock and offspring populations, and limited effective broodstock population size has contributed to the offspring. Both STRUCTURE and Principal Coordinates Analysis (PCoA) also showed the three populations composed of two stocks and most offspring individuals (95.0%) only originated from 44.0% of the individuals of broodstock I, which may have negative effects on sustainable fry production. Therefore, genetic variation between broodstock and offspring should be monitored, and large effective size of broodstock should be employed to ensure the success of commercial breeding programmes. Our data provide a useful genetic basis for future planning of sustainable culture and management of H. septemfasciatus. |
| 关键词 | Serranidae aquaculture genetic diversity genetic structure |
| DOI | 10.1017/S0025315416001260 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Fund[41506170] ; National Natural Science Fund[41276166] ; National Natural Science Fund[31672672] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; International Advanced Agricultural Science and Technology Plan[2014-Z49] ; Shandong Province agricultural seed project ; STS[KFJ-EW-STS-060-STS] ; Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, P.R. China ; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment[LFE-2015-8] ; Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Personnel of China[2013.01] ; North Jiangsu Science and technology development plan[BN2014107] ; National Natural Science Fund[41506170] ; National Natural Science Fund[41276166] ; National Natural Science Fund[31672672] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; International Advanced Agricultural Science and Technology Plan[2014-Z49] ; Shandong Province agricultural seed project ; STS[KFJ-EW-STS-060-STS] ; Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, P.R. China ; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment[LFE-2015-8] ; Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Personnel of China[2013.01] ; North Jiangsu Science and technology development plan[BN2014107] |
| WOS研究方向 | Marine & Freshwater Biology |
| WOS类目 | Marine & Freshwater Biology |
| WOS记录号 | WOS:000426876800005 |
| 出版者 | CAMBRIDGE UNIV PRESS |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158385 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Xiao, Zhizhong; Li, Jun |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Marine Biol Inst Shandong Prov, Qingdao 226104, Peoples R China 4.Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou 510300, Guangdong, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 通讯作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Xiao, Yongshuang,Guan, Shuguang,Liu, Qinghua,et al. Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers[J]. JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM,2018,98(2):261-267. |
| APA | Xiao, Yongshuang.,Guan, Shuguang.,Liu, Qinghua.,Liu, Hongjun.,Yu, Daode.,...&Li, Jun.(2018).Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers.JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM,98(2),261-267. |
| MLA | Xiao, Yongshuang,et al."Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers".JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM 98.2(2018):261-267. |
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| Genetic characterist（299KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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