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Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers
Xiao, Yongshuang1,2; Guan, Shuguang3; Liu, Qinghua1,2; Liu, Hongjun3; Yu, Daode3; Ma, Daoyuan1,2; Xu, Shihong1,2; Liu, Jing1; Dai, Ming4; Xiao, Zhizhong1,2; Li, Jun1,2
2018-03-01
Source PublicationJOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM
ISSN0025-3154
Volume98Issue:2Pages:261-267
Corresponding AuthorXiao, Zhizhong(xzz@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
AbstractSeven-band grouper (Hyporthodus septemfasciatus) is a commercial rocky reef fish in East Asia that has been regarded as a promising species for aquaculture. To investigate the broodstock contributions to offspring for the sustainability of fry production, 62 individuals of H. septemfasciatus from two broodstocks and one offspring population were analysed using fluorescent-AFLP. A total of 602 bands were amplified and 70.10% of them were polymorphic. The numbers of polymorphic loci were 308 (P-broodstock I = 55.50%) and 356 (P-broodstock II = 63.12%) in the two broodstocks, and 294 (P-offspring = 52.88%) in the offspring, respectively. The average values of Shannon diversity index (I) and expected heterozygosity (H) were higher in the broodstock (I-broodstock I = 0.281, I-broodstock II = 0.244, H-broodstock I = 0.185, H-broodstock II = 0.161) than those in the offspring (I-offspring = 0.243, H-offspring = 0.161). AMOVA and F-ST analyses showed that significant genetic differentiation between broodstock and offspring populations, and limited effective broodstock population size has contributed to the offspring. Both STRUCTURE and Principal Coordinates Analysis (PCoA) also showed the three populations composed of two stocks and most offspring individuals (95.0%) only originated from 44.0% of the individuals of broodstock I, which may have negative effects on sustainable fry production. Therefore, genetic variation between broodstock and offspring should be monitored, and large effective size of broodstock should be employed to ensure the success of commercial breeding programmes. Our data provide a useful genetic basis for future planning of sustainable culture and management of H. septemfasciatus.
KeywordSerranidae aquaculture genetic diversity genetic structure
DOI10.1017/S0025315416001260
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Fund[41506170] ; National Natural Science Fund[41276166] ; National Natural Science Fund[31672672] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; International Advanced Agricultural Science and Technology Plan[2014-Z49] ; Shandong Province agricultural seed project ; STS[KFJ-EW-STS-060-STS] ; Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, P.R. China ; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment[LFE-2015-8] ; Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Personnel of China[2013.01] ; North Jiangsu Science and technology development plan[BN2014107]
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000426876800005
PublisherCAMBRIDGE UNIV PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158385
Collection实验海洋生物学重点实验室
Corresponding AuthorXiao, Zhizhong; Li, Jun
Affiliation1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Marine Biol Inst Shandong Prov, Qingdao 226104, Peoples R China
4.Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou 510300, Guangdong, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xiao, Yongshuang,Guan, Shuguang,Liu, Qinghua,et al. Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers[J]. JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM,2018,98(2):261-267.
APA Xiao, Yongshuang.,Guan, Shuguang.,Liu, Qinghua.,Liu, Hongjun.,Yu, Daode.,...&Li, Jun.(2018).Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers.JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM,98(2),261-267.
MLA Xiao, Yongshuang,et al."Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers".JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM 98.2(2018):261-267.
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